Recombinant human Galectin 1 protein (ab50237)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceACGLVASNLN LKPGECLRVR GEVAPDAKSF VLNLGKDSNN LCLHFNPRFN AHGDANTIVC NSKDGGAWGT EQREAVFPFQ PGSVAEVCIT FDQANLTVKL PDGYEFKFPN RLNLEAINYM AADGDFKIKC VAFD

Specifications

Our Abpromise guarantee covers the use of ab50237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Endotoxin level< 0.100 Eu/µg
  • Purity> 95 % SDS-PAGE.

  • FormLyophilised
  • Additional notesDetermined by its ability to chemoattract human blood monocytes. Chemotactic activity was observed at a concentration of 2.5 µg/ml with a peak response obtained at 250 µg/ml.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Endotoxin level is less than 0.1 ng per µg (1EU/µg).

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionCentrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C; for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C; to -80°C;.

General Info

  • Alternative names
    • 14 kDa laminin-binding protein
    • 14 kDa lectin
    • Beta galactoside binding lectin
    • Beta galactoside binding lectin L 14 I
    • beta galactoside binding protein
    • Beta-galactoside-binding lectin L-14-I
    • DKFZp686E23103
    • Gal 1
    • Gal-1
    • GAL1
    • Galaptin
    • Galbp
    • Galectin
    • Galectin-1
    • Galectin1
    • GBP
    • HBL
    • HLBP14
    • HPL
    • L 14.5
    • L-14.5
    • L14
    • Lactose binding lectin 1
    • Lactose-binding lectin 1
    • Lect14
    • Lectin galactoside binding soluble 1
    • Lectin galactoside-binding soluble 1
    • LEG1_HUMAN
    • LGALS 1
    • LGALS1
    • Lgals1 lectin galactose binding soluble 1
    • MAPK activating protein MP12
    • Putative MAPK activating protein MP12
    • Putative MAPK-activating protein PM12
    • S Lac lectin 1
    • S-Lac lectin 1
    see all
  • FunctionMay regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase.
  • Tissue specificityExpressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.
  • Sequence similaritiesContains 1 galectin domain.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt

References for Recombinant human Galectin 1 protein (ab50237)

ab50237 has not yet been referenced specifically in any publications.

Product Wall

Application Functional Studies
Cells cultured in 6-well plates and in T-75 flasks were treated with 10µM galectin-1 (Abcam) in 1.2 mM DTT for 7 h at 37°C. The supernatant was removed and adherent cells on coverslips were rinsed with PBS and counter-stained with DAPI Mounting. Apoptotic nuclei were then visualized under a fluorescence microscope. For measuring caspase-3 activity, cells were collected with a plastic scraper, resuspended by gentle pipetting in PBS followed by protocol according to Millipore’s caspase-3 colorimetric activity assay kits. Fluorescence was measured at excitation at 400 nm and emission at 505 nm.

I find this product as good and acceptable for Galectin-1 prostate cell apoptosis assays.
Username

Dr. Armen Petrosyan

Verified customer

Submitted Oct 30 2014

Thank you for contacting us.

I recommend ab50237, based on test of the protein for chemotacttic activity, which is noted on the datasheet:

"Determined by its ability to chemoattract human blood monocytes. Chemotactic activity was ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"