Recombinant human Galectin 3 protein (ab78583)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceADNFSLHDAL SGSGNPNPQG WPGAWGNQPA GAGGYPGASY PGAYPGQAPP GAYPGQAPPG AYHGAPGAYP GAPAPGVYPG PPSGPGAYPS SGQPSAPGAY PATGPYGAPA GPLIVPYNLP LPGGVVPRML ITILGTVKPN ANRIALDFQR GNDVAFHFNP RFNENNRRVI VCNTKLDNNW GREERQSVFP FESGKPFKIQ VLVEPDHFKV AVNDAHLLQY NHRVKKLNEI SKLGISGDID LTSASYTMI
    • Molecular weight26 kDa
    • Amino acids1 to 250

Specifications

Our Abpromise guarantee covers the use of ab78583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    The activity is determined by the ability to induce chemotaxis of human PBMCs at concentrations ranging from 2-220 μg/mL. Corresponding to a specific activity of 5 x 102 units/mg.

  • Applications

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    Purity Approximately 97% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE. Endotoxin level as measured by LAL is <0.01ng/µg or <0.1EU/µg.
  • FormLyophilised
  • Additional notesFor long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 50mM Sodium chloride, 10mM Sodium phosphate, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionReconstitute with sterile water at 0.1-1.0 mg/ml, which can then be further diluted to other aqueous solutions.

General Info

  • Alternative names
    • 35 kDa lectin
    • Carbohydrate binding protein 35
    • Carbohydrate-binding protein 35
    • CBP 35
    • CBP35
    • Gal-3
    • GAL3
    • Galactose-specific lectin 3
    • Galactoside-binding protein
    • GALBP
    • Galectin 3 internal gene,included
    • Galectin-3
    • Galectin3
    • GALIG
    • GBP
    • IgE binding protein
    • IgE-binding protein
    • L 31
    • L 34
    • L-31
    • L-34 galactoside-binding lectin
    • L31
    • Laminin-binding protein
    • Lectin L-29
    • Lectin, galactose binding, soluble 3
    • LEG3_HUMAN
    • LGALS2
    • LGALS3
    • MAC 2 antigen
    • Mac-2
    • Mac-2 antigen
    • MAC2
    • Macrophage galactose-specific lectin
    • MGC105387
    see all
  • FunctionGalactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis.
  • Tissue specificityA major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.
  • Sequence similaritiesContains 1 galectin domain.
  • Cellular localizationNucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface.
  • Information by UniProt

Recombinant human Galectin 3 protein images

  • ab78583 used in Western Blot. Figure: 1 ug under (+) reducing and (-) non-reducing conditions in a 4-20% Tris-Glycine gel stained with Coomassie Blue. Human Galectin-3 has a predicted MW of 26 kDa.

References for Recombinant human Galectin 3 protein (ab78583)

ab78583 has not yet been referenced specifically in any publications.

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We recommend a starting concentration of 0.1ug/ml when using purified antibodies in ELISA. This will have to optimized for your specific protocol.

Should you need a positive control for your ELISA we...

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