The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% SDS-PAGE. ab117677 was purified by proprietary chromatographic techniques and filter sterilized.
Additional notesStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
FunctionBinds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. It is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death. Isoform Short acts as an eosinophil chemoattractant.
Tissue specificityPeripheral blood leukocytes and lymphatic tissues. Overexpressed in Hodgkin disease tissue.
Sequence similaritiesContains 2 galectin domains.
DomainContains two homologous but distinct carbohydrate-binding domains.
Cellular localizationCytoplasm. Secreted. May also be secreted by a non-classical secretory pathway.