Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MKHHHHHHAS AAIPVPKLSC KNLCHRHQLF INFRDLGWHK WIIAPKGFMA NYCHGECPFS LTISLNSSNY AFMQALMHAV DPEIPQAVCI PTKLSPISML YQDNNDNVIL RHYEDMVVDECGCG
    • Molecular weight
      14 kDa including tags
    • Amino acids
      251 to 364
    • Tags
      His tag N-Terminus
    • Additional sequence information
      GDF3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 124 amino acids and having a total molecular mass of 14.15 kDa.

Specifications

Our Abpromise guarantee covers the use of ab117474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity
    > 90 % SDS-PAGE.
    ab117474 is purified by proprietary chromatographic techniques.
  • Form
    Lyophilised
  • Additional notes
    Lyophilized ab117474, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution ab117474 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Please see notes section.

  • Reconstitution
    It is recommended to reconstitute in sterile 100mM Acetate buffer pH4 at a concentration of 0.5mg/ml. For the dilution into higher pH values, it is recommended to dilute the protein to a concentration of 10µg/ml. Please note that in higher concentrations the solubility of ab117474 is limited. The protein is not sterile! Please sterile filter before using it in the cell culture.

General Info

  • Alternative names
    • C78318
    • ecat9
    • GDF 3
    • GDF-3
    • GDF3
    • GDF3_HUMAN
    • Growth differentiation factor 3
    • Growth/differentiation factor 3
    • KFS3
    • MCOP7
    • MCOPCB6
    • MGC123990
    • MGC123991
    • RGD1564178
    • Vgr 2
    • Vgr2
    see all
  • Sequence similarities
    Belongs to the TGF-beta family.
  • Cellular localization
    Secreted.
  • Information by UniProt

References for Recombinant Human GDF3 protein (ab117474)

ab117474 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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