Recombinant human GDF8 / Myostatin protein (ab73251)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionO14793
    • SpeciesHuman
    • SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Asp-Phe-Gly-Leu-Asp.
    • Additional sequence informationThe sequence of the first five N-terminal amino acids was determined and was found to be Asp-Phe-Gly-Leu-Asp so starting at aa 267.

Associated products

Specifications

Our Abpromise guarantee covers the use of ab73251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    The ED50 as determined by the inhibition of the proliferation of MPC-11 cells is < 20ng/ml, corresponding to a Specific Activity of 50,000units/mg.

  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    This product is purified by proprietary chromatographic techniques. Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
  • FormLyophilised
  • Additional notesFor long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None.
    Additives: None.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionReconstitute in sterile 20mM HCl at 0.1 mg/ml, which can then be further diluted to other aqueous solutions.

General Info

  • Alternative names
    • GDF 8
    • GDF-8
    • GDF8
    • GDF8_HUMAN
    • Growth differentiation factor 8
    • Growth/differentiation factor 8
    • MSLHP
    • MSTN
    • Myostatin
    • OTTHUMP00000163498
    see all
  • FunctionActs specifically as a negative regulator of skeletal muscle growth.
  • Sequence similaritiesBelongs to the TGF-beta family.
  • Cellular localizationSecreted.
  • Information by UniProt

References for Recombinant human GDF8 / Myostatin protein (ab73251)

ab73251 has not yet been referenced specifically in any publications.

Product Wall

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We would suggest to reconstitute the sample in 20 mM HCL prior to diluting it. For our assay, the myostatin was not replenished everyday. Also, we are not sure of the stability in culture, but our assays are run f...

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Thank you for contacting us.

These two proteins are the same, except for the reconstitution instructions (water versus 20 mM Hcl). Both should work equally well in inhiibiting MPC-11 cell growth.

I hope this information is helpful to ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"