Recombinant human GLO1 protein (ab87413)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceMAEPQPPSGG LTDEAALSCC SDADPSTKDF LLQQTMLRVK DPKKSLDFYT RVLGMTLIQK CDFPIMKFSL YFLAYEDKND IPKEKDEKIA WALSRKATLE LTHNWGTEDD ETQSYHNGNS DPRGFGHIGI AVPDVYSACK RFEELGVKFV KKPDDGKMKG LAFIQDPDGY WIEILNPNKM ATLM

Specifications

Our Abpromise guarantee covers the use of ab87413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activitySpecific activity: >0.4 units/mg (please enquire for specific batch value). One unit will form 1.0 µmol of S-lactoylglutathione from methylglyoxal and reduced glutathione per minute at pH 7.5, at 25°C. Specific activity was expressed in units/mg of protein.

  • Applications

    SDS-PAGE

    Functional Studies

  • Purity> 90 % SDS-PAGE.
    Purified by using conventional chromatography techniques.
  • FormLiquid
  • Additional notesActivity Assay
  • FInal assay concentrations:In 1.5ml reaction mix, the final concentrations are 79 mM potassium phosphate, 0.033%(w/v) reduced glutathine, 0.003% (w/v) bovine serum albumin, 0.033% methylglyoxal(w/v) and recombinant gyloxalase l (0.5ug, 1ug, 2ug).
  • 1. Prepare protein-free reaction buffer without recombinant protein into suitable container (for each protein concentration).
  • 2. Equilibrate protein-free reaction buffer to 25°C and monitor at A240nm (absorbance) until the value is constant, using a spectrophotometer.
  • 3. Dilute various concentrations of recombinant gyloxalase l (0.5ug, 1ug, 2ug) in 50ul of 10mM potassium phosphate buffer with 0.1% (wv) BSA, pH7.4.
  • Add 50ul of recombinant glyoxalase I solution to 1.45ml reaction buffer.
  • Immediately mix by inversion and record the increase at A240nm for 5 minutes
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 1mM DTT, pH 8

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Aldoketomutase
    • glo1
    • GLOD1
    • Glx I
    • GLYI
    • glyoxalase domain containing 1
    • Glyoxalase I
    • Ketone aldehyde mutase
    • Ketone-aldehyde mutase
    • Lactoyl glutathione lyase
    • Lactoylglutathione lyase
    • LGUL_HUMAN
    • Methylglyoxalase
    • S D lactoylglutathione methylglyoxal lyase
    • S-D-lactoylglutathione methylglyoxal lyase
    see all
  • FunctionCatalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione.
  • PathwaySecondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2.
  • Sequence similaritiesBelongs to the glyoxalase I family.
  • Information by UniProt

Recombinant human GLO1 protein images

  • ab87413 on 15% SDS-PAGE (3µg)

References for Recombinant human GLO1 protein (ab87413)

ab87413 has not yet been referenced specifically in any publications.

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