Recombinant human GLO1 protein (ab87413)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      MAEPQPPSGG LTDEAALSCC SDADPSTKDF LLQQTMLRVK DPKKSLDFYT RVLGMTLIQK CDFPIMKFSL YFLAYEDKND IPKEKDEKIA WALSRKATLE LTHNWGTEDD ETQSYHNGNS DPRGFGHIGI AVPDVYSACK RFEELGVKFV KKPDDGKMKG LAFIQDPDGY WIEILNPNKM ATLM

Specifications

Our Abpromise guarantee covers the use of ab87413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific activity: >0.4 units/mg (please enquire for specific batch value). One unit will form 1.0 µmol of S-lactoylglutathione from methylglyoxal and reduced glutathione per minute at pH 7.5, at 25°C. Specific activity was expressed in units/mg of protein.

  • Applications

    SDS-PAGE

    Functional Studies

  • Purity
    > 90 % SDS-PAGE.
    Purified by using conventional chromatography techniques.
  • Form
    Liquid
  • Additional notes
    Activity Assay
  • FInal assay concentrations:In 1.5ml reaction mix, the final concentrations are 79 mM potassium phosphate, 0.033%(w/v) reduced glutathine, 0.003% (w/v) bovine serum albumin, 0.033% methylglyoxal(w/v) and recombinant gyloxalase l (0.5ug, 1ug, 2ug).
  • 1. Prepare protein-free reaction buffer without recombinant protein into suitable container (for each protein concentration).
  • 2. Equilibrate protein-free reaction buffer to 25°C and monitor at A240nm (absorbance) until the value is constant, using a spectrophotometer.
  • 3. Dilute various concentrations of recombinant gyloxalase l (0.5ug, 1ug, 2ug) in 50ul of 10mM potassium phosphate buffer with 0.1% (wv) BSA, pH7.4.
  • Add 50ul of recombinant glyoxalase I solution to 1.45ml reaction buffer.
  • Immediately mix by inversion and record the increase at A240nm for 5 minutes
  • Concentration information loading...
  • Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      Preservative: None
      Constituents: 10% Glycerol, 20mM Tris HCl, 1mM DTT, pH 8

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    General Info

    • Alternative names
      • Aldoketomutase
      • glo1
      • GLOD1
      • Glx I
      • GLYI
      • glyoxalase domain containing 1
      • Glyoxalase I
      • Ketone aldehyde mutase
      • Ketone-aldehyde mutase
      • Lactoyl glutathione lyase
      • Lactoylglutathione lyase
      • LGUL_HUMAN
      • Methylglyoxalase
      • S D lactoylglutathione methylglyoxal lyase
      • S-D-lactoylglutathione methylglyoxal lyase
      see all
    • Function
      Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione.
    • Pathway
      Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2.
    • Sequence similarities
      Belongs to the glyoxalase I family.
    • Information by UniProt

    Images

    • ab87413 on 15% SDS-PAGE (3µg)

    References

    ab87413 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Thank you very much for your call yesterday and for your patience while I have been in touch with the lab regarding your enquiry:

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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