Recombinant Human Glucokinase protein (ab119445)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MGSSHHHHHHSSGLVPRGSHMGSHMAAPGALLVMGVSGSGKSTVGALLAS ELGWKFYDADDYHPEENRRKMGKGIPLNDQDRIPWLCNLHDILLRDVASG QRVVLACSALKKTYRDILTQGKDGVALKCEESGKEAKQAEMQLLVVHLSG SFEVISGRLLKREGHFMPPELLQSQFETLEPPAAPENFIQISVDKNVSEI IATIMETLKMK
    • Molecular weight
      23 kDa including tags
    • Amino acids
      1 to 187
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab119445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity
    > 95 % SDS-PAGE.
    ab119445 was purified using conventional chromatography techniques.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.02% DTT, 0.32% Tris HCl, 20% Glycerol, 0.58% Sodium chloride

General Info

  • Alternative names
    • ATP:D-hexose 6-phosphotransferase
    • FGQTL3
    • GCK
    • GK
    • GLK
    • Glucokinase
    • Hexokinase D pancreatic isozyme
    • Hexokinase type IV
    • Hexokinase-4
    • Hexokinase-D
    • HHF3
    • HK IV
    • HK4
    • HKIV
    • HXK4_HUMAN
    • HXKP
    • LGLK
    • MODY2
    see all
  • Function
    Catalyzes the initial step in utilization of glucose by the beta-cell and liver at physiological glucose concentration. Glucokinase has a high Km for glucose, and so it is effective only when glucose is abundant. The role of GCK is to provide G6P for the synthesis of glycogen. Pancreatic glucokinase plays an important role in modulating insulin secretion. Hepatic glucokinase helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage.
  • Tissue specificity
    Isoform 1 is expressed in pancreas. Isoform 2 and isoform 3 is expressed in liver.
  • Involvement in disease
    Defects in GCK are the cause of maturity-onset diabetes of the young type 2 (MODY2) [MIM:125851]; also shortened MODY-2. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
    Defects in GCK are the cause of familial hyperinsulinemic hypoglycemia type 3 (HHF3) [MIM:602485]; also known as persistent hyperinsulinemic hypoglycemia of infancy (PHHI) or congenital hyperinsulinism. HHF is the most common cause of persistent hypoglycemia in infancy. Unless early and aggressive intervention is undertaken, brain damage from recurrent episodes of hypoglycemia may occur.
  • Sequence similarities
    Belongs to the hexokinase family.
  • Information by UniProt

Images

  • 12% SDS-PAGE showing ab119445 at approximately 23.1kDa (3µg).

References

ab119445 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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