Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.
Involvement in disease
Defects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency (HA-GPID) [MIM:613470]. It is a form of anemia in which there is no abnormal hemoglobin or spherocytosis. It is caused by glucose phosphate isomerase deficiency. Severe GPI deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.
Belongs to the GPI family.
Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway. ISGylated.
SDS-PAGE - Glucose 6 phosphate isomerase protein (His tag) (ab87625)
15% SDS-PAGE showing ab87625 at approximately 65kDa (3µg).
Western blot - Anti-Glucose 6 phosphate isomerase antibody (ab86950)
All lanes : Anti-Glucose 6 phosphate isomerase antibody (ab86950) at 1 µg/ml
Lane 1 : Recombinant Human Glucose 6 phosphate isomerase protein (ab87625) at 0.1 µg Lane 2 : Recombinant Human Glucose 6 phosphate isomerase protein (ab87625) at 0.01 µg
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution Developed using the ECL technique
Performed under reducing conditions.
Exposure time : 30 seconds
This product has been referenced in:
Tian K et al. Microfluidics-based optimization of neuroleukin-mediated regulation of articular chondrocyte proliferation. Mol Med Rep13:67-74 (2016).
Read more (PubMed: 26573126) »
Zong M et al. Glucose-6-phosphate isomerase promotes the proliferation and inhibits the apoptosis in fibroblast-like synoviocytes in rheumatoid arthritis. Arthritis Res Ther17:100 (2015).
Read more (PubMed: 25889507) »