Recombinant Human Glutamine Synthetase protein (ab98145)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MGSSHHHHHHSSGLVPRGSHMTTSASSHLNKGIKQVYMSLPQGEKVQAMY IWIDGTGEGLRCKTRTLDSEPKCVEELPEWNFDGSSTLQSEGSNSDMYLV PAAMFRDPFRKDPNKLVLCEVFKYNRRPAETNLRHTCKRIMDMVSNQHPW FGMEQEYTLMGTDGHPFGWPSNGFPGPQGPYYCGVGADRAYGRDIVEAHY RACLYAGVKIAGTNAEVMPAQWEFQIGPCEGISMGDHLWVARFILHRVCE DFGVIATFDPKPIPGNWNGAGCHTNFSTKAMREENGLKYIEEAIEKLSKR HQYHIRAYDPKGGLDNARRLTGFHETSNINDFSAGVANRSASIRIPRTVG QEKKGYFEDRRPSANCDPFSVTEALIRTCLLNETGDEPFQYKN
    • Molecular weight
      44 kDa including tags
    • Amino acids
      1 to 373
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab98145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry

    SDS-PAGE

    Western blot

  • Mass spectrometry
    MALDI-TOF
  • Purity
    > 90 % SDS-PAGE.
    ab98145 was purified using conventional chromatography techniques.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 20% Glycerol, 5mM DTT, 200mM Sodium chloride, 20mM Tris HCl, pH 8.0

General Info

  • Alternative names
    • cell proliferation-inducing protein 59
    • GLNA
    • GLNA_HUMAN
    • GLNS
    • GLUL
    • Glutamate ammonia ligase
    • Glutamate decarboxylase
    • Glutamate--ammonia ligase
    • glutamine synthase
    • Glutamine synthetase
    • GS
    • PIG 43
    • PIG 59
    • PIG43
    • PIG59
    • Proliferation inducing protein 43
    see all
  • Function
    This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts.
  • Involvement in disease
    Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid.
  • Sequence similarities
    Belongs to the glutamine synthetase family.
  • Developmental stage
    Expressed during early fetal stages.
  • Cellular localization
    Cytoplasm. Mitochondrion.
  • Information by UniProt

Recombinant Human Glutamine Synthetase protein images

  • 15% SDS-PAGE analysis of 3µg ab98145

References for Recombinant Human Glutamine Synthetase protein (ab98145)

ab98145 has not yet been referenced specifically in any publications.

Product Wall

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I'm sending a free of charge vial on the order ***, which should arrive tomorrow.

Please keep me updated about the results with this new vial. If there is anything else that we can do for you, ple...

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Thank you again for letting us know about the trouble with this protein, and for your patience while the lab has worked to determine the cause of this problem. I apologize for the delay in resolving this.

The protein has been re-tested (pleas...

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Thank you again for letting us know about the trouble with this protein and for your patience.

The lab is re-testing the protein that is in stock and we should have the results shortly. I will let you know the results of these tests as soon a...

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Thank you very much for your email and for sending the data.

I have forwarded this on to the lab and asked if they know the identity of these bands, andI've also enquired whetherthere are any other lots that do not show these extra bands. Read More

Thank you very much for your call today and for letting us know about the trouble with this protein.

As we discussed, if you have an image of the results it would be very helpful for us, and also if you could send me the lot number I can ask ...

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