Recombinant Human Glutaredoxin 1 protein (ab86987)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      MAQEFVNCKI QPGKVVVFIK PTCPYCRRAQ EILSQLPIKQ GLLEFVDITA TNHTNEIQDY LQQLTGARTV PRVFIGKDCI GGCSDLVSLQ QSGELLTRLK QIGALQ

Associated products

Specifications

Our Abpromise guarantee covers the use of ab86987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.
    ab86987 is purified using conventional chromatography techniques.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 1mM DTT, pH 8.0

General Info

  • Alternative names
    • GLRX
    • Glrx1
    • GLRX1_HUMAN
    • Glutaredoxin
    • Glutaredoxin-1
    • Glutaredoxin1
    • Grx
    • Grx 1
    • Grx1
    • MGC117407
    • Thioltransferase
    • Thioltransferase 1
    • Thioltransferase-1
    • Thioltransferase1
    • TTase
    • TTase 1
    • TTase-1
    • TTase1
    see all
  • Function
    Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins.
  • Sequence similarities
    Belongs to the glutaredoxin family.
    Contains 1 glutaredoxin domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt

Recombinant Human Glutaredoxin 1 protein images

  • 15% SDS-PAGE showing ab86987 at approximately 11.7kDa (3µg).
  • All lanes : Anti-Glutaredoxin 1 antibody (ab45953) at 1/250 dilution

    Lane 1 : Recombinant Human Glutaredoxin 1 protein (ab86987) at 0.01 µg
    Lane 2 : Recombinant Human Glutaredoxin 1 protein (ab86987) at 0.001 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

References for Recombinant Human Glutaredoxin 1 protein (ab86987)

ab86987 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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