Recombinant human Glutaredoxin 2 protein (ab82665)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • Amino acids41 to 164

Specifications

Our Abpromise guarantee covers the use of ab82665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activitySpecific activity : 45 U/mg One unit will cause the oxidation of 1µmole of NADPH per min.
  • Applications

    Functional Studies

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 20mM HEPES, pH 7

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • bA101E13.1
    • bA101E13.1 (GRX2 glutaredoxin (thioltransferase) 2)
    • CGI133
    • Glrx2
    • GLRX2_HUMAN
    • Glutaredoxin-2
    • Glutaredoxin-2, mitochondrial
    • GRX2
    • mitochondrial
    see all
  • FunctionGlutathione-dependent oxidoreductase that facilitates the maintenance of mitochondrial redox homeostasis upon induction of apoptosis by oxidative stress. Involved in response to hydrogen peroxide and regulation of apoptosis caused by oxidative stress. Acts as a very efficient catalyst of monothiol reactions because of its high affinity for protein glutathione-mixed disulfides. Can receive electrons not only from glutathione (GSH), but also from thioredoxin reductase supporting both monothiol and dithiol reactions. Efficiently catalyzes both glutathionylation and deglutathionylation of mitochondrial complex I, which in turn regulates the superoxide production by the complex. Overexpression decreases the susceptibility to apoptosis and prevents loss of cardiolipin and cytochrome c release.
  • Tissue specificityWidely expressed. Expressed in brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta and lung. Not expressed in peripheral blood leukocytes.
  • Sequence similaritiesBelongs to the glutaredoxin family.
    Contains 1 glutaredoxin domain.
  • Cellular localizationMitochondrion and Nucleus.
  • Information by UniProt

Recombinant human Glutaredoxin 2 protein images

  • ab82665 at 1µg on SDS-PAGE.

References for Recombinant human Glutaredoxin 2 protein (ab82665)

ab82665 has not yet been referenced specifically in any publications.

Product Wall

Here is how the lab performs the activity assay:


100 mM Tris-Cl(pH8.0), 2 mM EDTA, 0.1 mg/ml BSA, 1 mM GSH, 0.2 mM NADPH, 6 ug/ml yeast GR, 0.7 mM HED and X ug Grx2 protein.


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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"