The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityab83993 bound to protein A sepharose beads was able to pull down its ligand, GM-CSF.
Additional notesab83993 bound to protein A sepharose beads was able to pull down its ligand, GM-CSF.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C.
Constituents: 10% Trehalose, 1% Human serum albumin
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionIt is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
FunctionLow affinity receptor for granulocyte-macrophage colony-stimulating factor. Transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells.
Involvement in diseaseDefects in CSF2RA are the cause of pulmonary surfactant metabolism dysfunction type 4 (SMDP4) [MIM:300770]. A rare lung disorder due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress.
Sequence similaritiesBelongs to the type I cytokine receptor family. Type 5 subfamily.
DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box 1 motif is required for JAK interaction and/or activation.
Recombinant human GM-CSF Receptor alpha protein (Fc Chimera) images
Functional Studies - GM-CSF Receptor alpha - Fc Chimera (ab83993)
Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational
The triangle indicates the theoretical MW and pI of the protein.
SDS-PAGE - GM-CSF Receptor alpha - Fc Chimera (ab83993)
1D SDS-PAGE of ab83993 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab83993
Lane 2: ab83993 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab83993 treated with a glycosidase
cocktail to remove potential N- and O-linked glycans. Approximately 5 μg of protein was loaded per lane; Gel was stained using Deep Purple™.
Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. A tightening of the band after treatment with the glycosidase cocktail indicates O-linked glycans may be present. Additional bands in lane 2 and lane 3 are glycosidase enzymes.
References for Recombinant human GM-CSF Receptor alpha protein (Fc Chimera) (ab83993)
has not yet been referenced specifically in any publications.
Publishing research using ab83993? Please let us know so that we can cite the reference in this datasheet.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"