The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Additional notesBest use within three months from the date of receipt of this protein.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00 Constituents: 0.79% Tris HCl, 0.31% Glutathione
Golgi peripheral membrane protein p65
Golgi phosphoprotein 5
Golgi reassembly and stacking protein 1
Golgi reassembly and stacking protein 65 kDa
Golgi reassembly and stacking protein, 65-kD
Golgi reassembly stacking protein 1
Golgi reassembly stacking protein 1 65kDa
Golgi reassembly stacking protein of 65 kDa
Golgi reassembly-stacking protein 1
Golgi reassembly-stacking protein of 65 kDa
FunctionStacking factor involved in the postmitotic assembly of Golgi stacks from mitotic Golgi fragments. Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis. Also mediates, via its interaction with GM130, the docking of transport vesicles with the Golgi membranes.
Sequence similaritiesBelongs to the GORASP family. Contains 1 PDZ (DHR) domain.
Post-translational modificationsPhosphorylated by CDC2/B1 and PLK kinases during mitosis. Phosphorylation cycle correlates with the cisternal stacking cycle. Phosphorylation of the homodimer prevents the association of dimers into higher order oligomers, leading to cisternal unstacking. Target for caspase-3 cleavage during apoptosis. The cleavage contributes to Golgi fragmentation and occurs very early in the execution phase of apoptosis.
Cellular localizationGolgi apparatus > cis-Golgi network membrane. Undergoes rapid exchange with the cytosol.