Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human

Specifications

Our Abpromise guarantee covers the use of ab85245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

  • Purity
    > 95 % SDS-PAGE.
    Purity was determined to be >95% by densitometry, SDS image.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM PMSF, pH 7.5

General Info

  • Alternative names
    • Abundant SRC homology
    • Adapter protein GRB2
    • ASH
    • Ash protein
    • EGFRBP GRB2
    • Epidermal growth factor receptor binding protein
    • Epidermal growth factor receptor binding protein GRB2
    • GRB 2
    • Grb2
    • GRB2 adapter protein
    • GRB2_HUMAN
    • Grb3 3
    • Growth factor receptor bound protein 2
    • Growth factor receptor bound protein 3
    • Growth factor receptor-bound protein 2
    • HT027
    • MST084
    • MSTP084
    • NCKAP2
    • OTTHUMP00000166096
    • OTTHUMP00000166097
    • OTTHUMP00000166098
    • Protein ash
    • SEM5
    • SH2/SH3 adapter GRB2
    see all
  • Function
    Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
    Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.
  • Sequence similarities
    Belongs to the GRB2/sem-5/DRK family.
    Contains 1 SH2 domain.
    Contains 2 SH3 domains.
  • Domain
    The SH3 domains mediate interaction with RALGPS1 and SHB.
  • Cellular localization
    Golgi apparatus.
  • Information by UniProt

Images

  • SDS-PAGE showing ab85245 at approximately 49kDa.

References

ab85245 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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