Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      95 kDa
    • Amino acids
      1 to 563

Specifications

Our Abpromise guarantee covers the use of ab105126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific activity: 4.3 nmol/min/mg
  • Applications

    Western blot

    Functional Studies

  • Purity
    > 95 % SDS-PAGE.

  • Form
    Liquid
  • Additional notes

    It is recommended to store this protein at -80°C in glycerol to preserve ts activity.

     

    ab204870 (Cdc25C peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • EC=2.7.11.14
    • G protein-coupled receptor kinase 1
    • GPRK1
    • Grk1
    • Rhodopsin kinase
    • RHOK
    • RK
    • RK_HUMAN
    see all
  • Function
    Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade. This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination.
  • Tissue specificity
    Retinal-specific. Expressed in rods and cones cells.
  • Involvement in disease
    Night blindness, congenital stationary, Oguchi type 2
  • Sequence similarities
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
    Contains 1 RGS domain.
  • Post-translational
    modifications
    Autophosphorylated, Ser-21 is a minor site of autophosphorylation compared to Ser-491 and Thr-492 (By similarity). Phosphorylation at Ser-21 is regulated by light and activated by cAMP.
    Farnesylation is required for full activity.
  • Cellular localization
    Membrane.
  • Information by UniProt

Images

  • Kinase Activity Plot. Specific activity is 4.3 nmol/min/mg
  • Recombinant human GRK1 protein (ab105126) + plus proprietary tag

References

ab105126 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab105126.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up