Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • AccessionQ15835
    • SpeciesHuman
    • Molecular weight95 kDa
    • Amino acids1 to 563

Specifications

Our Abpromise guarantee covers the use of ab105126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activitySpecific activity: 4.3 nmol/min/mg
  • Applications

    Western blot

    Functional Studies

  • Purity> 95 % SDS-PAGE.

  • FormLiquid
  • Additional notes

    It is recommended to store this protein at -80°C in glycerol to preserve ts activity.

     

    ab204870 (Cdc25C peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • EC=2.7.11.14
    • G protein-coupled receptor kinase 1
    • GPRK1
    • Grk1
    • Rhodopsin kinase
    • RHOK
    • RK
    • RK_HUMAN
    see all
  • FunctionRetina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade. This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination.
  • Tissue specificityRetinal-specific. Expressed in rods and cones cells.
  • Involvement in diseaseNight blindness, congenital stationary, Oguchi type 2
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
    Contains 1 RGS domain.
  • Post-translational
    modifications
    Autophosphorylated, Ser-21 is a minor site of autophosphorylation compared to Ser-491 and Thr-492 (By similarity). Phosphorylation at Ser-21 is regulated by light and activated by cAMP.
    Farnesylation is required for full activity.
  • Cellular localizationMembrane.
  • Information by UniProt

Recombinant human GRK1 protein images

  • Kinase Activity Plot. Specific activity is 4.3 nmol/min/mg
  • Recombinant human GRK1 protein (ab105126) + plus proprietary tag

References for Recombinant human GRK1 protein (ab105126)

ab105126 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"