Recombinant Human GRP94 protein (ab87144)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceMRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMDDE VDVDGTVEED LGKSREGSRT DDEVVQREEE AIQLDGLNAS QIRELREKSE KFAFQAEVNR MMKLIINSLY KNKEIFLREL ISNASDALDK IRLISLTDEN ALSGNEELTV KIKCDKEKNL LHVTDTGVGM TREELVKNLG TIAKSGTSEF LNKMTEAQED GQSTSELIGQ FGVGFYSAFL VADKVIVTSK HNNDTQHIWE SDSNEFSVIA DPRGNTLGRG TTITLVLKEE ASDYLELDTI KNLVKKYSQF INFPIYVWSS KTETVEEPME EEEAAKEEKE ESDDEAAVEE EEEEKKPKTK KVEKTVWDWE LMNDIKPIWQ RPSKEVEEDE YKAFYKSFSK ESDDPMAYIH FTAEGEVTFK SILFVPTSAP RGLFDEYGSK KSDYIKLYVR RVFITDDFHD MMPKYLNFVK GVVDSDDLPL NVSRETLQQH KLLKVIRKKL VRKTLDMIKK IADDKYNDTF WKEFGTNIKL GVIEDHSNRT RLAKLLRFQS SHHPTDITSL DQYVERMKEK QDKIYFMAGS SRKEAESSPF VERLLKKGYE VIYLTEPVDE YCIQALPEFD GKRFQNVAKE GVKFDESEKT KESREAVEKE FEPLLNWMKD KALKDKIEKA VVSQRLTESP CALVASQYGW SGNMERIMKA QAYQTGKDIS TNYYASQKKT FEINPRHPLI RDMLRRIKED EDDKTVLDLA VVLFETATLR SGYLLPDTKA YGDRIERMLR LSLNIDPDAK VEEEPEEEPE ETAEDTTEDT EQDEDEEMDV

Specifications

Our Abpromise guarantee covers the use of ab87144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

  • Purity> 90 % SDS-PAGE.
    ab87144 was purified using conventional chromatography
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, 1mM EDTA, 1mM DTT, pH 8.0

General Info

  • Alternative names
    • 94 kDa glucose regulated protein
    • 94 kDa glucose-regulated protein
    • ECGP
    • Endoplasmin
    • Endothelial cell (HBMEC) glycoprotein
    • ENPL_HUMAN
    • Glucose regulated protein 94kDa
    • gp96
    • gp96 homolog
    • GRP 94
    • GRP-94
    • Heat shock protein 90 kDa beta member 1
    • heat shock protein 90kDa beta (Grp94), member 1
    • Heat shock protein, 90 kDa, beta, 1
    • HSP90B1
    • Stress inducible tumor rejection antigen GP96
    • TRA1
    • tumor rejection antigen (gp96) 1
    • Tumor rejection antigen 1
    • Tumor rejection antigen gp96
    • Tumor rejection antigen-1 (gp96)
    see all
  • FunctionMolecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.
  • Sequence similaritiesBelongs to the heat shock protein 90 family.
  • Cellular localizationEndoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

Recombinant Human GRP94 protein images

  • 15% SDS-PAGE showing ab87144 (3ug) at 94.4 kDa
  • Anti-GRP94 antibody (ab13509) at 2 µg/ml + Recombinant Human GRP94 protein (ab87144) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 150 seconds

References for Recombinant Human GRP94 protein (ab87144)

ab87144 has not yet been referenced specifically in any publications.

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