Recombinant Human GRP94 protein (Tagged-His Tag) (ab188463)

Overview

Description

  • Nature
    Recombinant
  • Source
    HEK 293 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      ASDDEVDVDG TVEEDLGKSR EGSRTDDEVV QREEEAIQLD GLNASQIREL REKSEKFAFQ AEVNRMMKLI INSLYKNKEI FLRELISNAS DALDKIRLIS LTDENALSGN EELTVKIKCD KEKNLLHVTD TGVGMTREEL VKNLGTIAKS GTSEFLNKMT EAQEDGQSTS ELIGQFGVGF YSAFLVADKV IVTSKHNNDT QHIWESDSNE FSVIADPRGN TLGRGTTITL VLKEEASDYL ELDTIKNLVK KYSQFINFPI YVWSSKTETV EEPMEEEEAA KEEKEESDDE AAVEEEEEEK KPKTKKVEKT VWDWELMNDI KPIWQRPSKE VEEDEYKAFY KSFSKESDDP MAYIHFTAEG EVTFKSILFV PTSAPRGLFD EYGSKKSDYI KLYVRRVFIT DDFHDMMPKY LNFVKGVVDS DDLPLNVSRE TLQQHKLLKV IRKKLVRKTL DMIKKIADDK YNDTFWKEFG TNIKLGVIED HSNRTRLAKL LRFQSSHHPT DITSLDQYVE RMKEKQDKIY FMAGSSRKEA ESSPFVERLL KKGYEVIYLT EPVDEYCIQA LPEFDGKRFQ NVAKEGVKFD ESEKTKESRE AVEKEFEPLL NWMKDKALKD KIEKAVVSQR LTESPCALVA SQYGWSGNME RIMKAQAYQT GKDISTNYYA SQKKTFEINP RHPLIRDMLR RIKEDEDDKT VLDLAVVLFE TATLRSGYLL PDTKAYGDRI ERMLRLSLNI DPDAKVEEEP EEEPEETAED TTEDTEQDED EEMDVGTDEE ETAKESTAE G PKLHHHHHH
    • Molecular weight
      91 kDa including tags
    • Amino acids
      22 to 798
    • Tags
      His tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab188463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

    Mass Spectrometry

    SDS-PAGE

  • Endotoxin level
    < 1.000 Eu/µg
  • Mass spectrometry
    LC-MS/MS
  • Purity
    >90% by SDS-PAGE.

  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 95% PBS, 1% Sucrose, 4% Mannitol

  • Reconstitution
    Add 200 µl of deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.

General Info

  • Alternative names
    • 94 kDa glucose regulated protein
    • 94 kDa glucose-regulated protein
    • ECGP
    • Endoplasmin
    • Endothelial cell (HBMEC) glycoprotein
    • ENPL_HUMAN
    • Glucose regulated protein 94kDa
    • gp96
    • gp96 homolog
    • GRP 94
    • GRP-94
    • Heat shock protein 90 kDa beta member 1
    • heat shock protein 90kDa beta (Grp94), member 1
    • Heat shock protein, 90 kDa, beta, 1
    • HSP90B1
    • Stress inducible tumor rejection antigen GP96
    • TRA1
    • tumor rejection antigen (gp96) 1
    • Tumor rejection antigen 1
    • Tumor rejection antigen gp96
    • Tumor rejection antigen-1 (gp96)
    see all
  • Function
    Molecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.
  • Sequence similarities
    Belongs to the heat shock protein 90 family.
  • Cellular localization
    Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

Images

  • 14 % SDS-PAGE separation of Human GRP94:
    1. MW marker - 14, 21, 31, 45, 66, 97 kDa
    2. Reduced and boiled sample, 2.5 μg/lane
    3. Non-reduced and non-boiled sample, 2.5 μg/lane

References

ab188463 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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