Recombinant human GSK3 beta protein (Active) (ab60863)

Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      73 kDa
    • Tags
      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab60863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Specific Activity: 187 nmol/min/mg

  • Applications

    SDS-PAGE

    Functional Studies

    Western blot

  • Form
    Liquid
  • Additional notes

    ab204860 (Glycogen synthase 1 peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Glycogen Synthase Kinase 3 Beta
    • Glycogen synthase kinase-3 beta
    • GSK 3 beta
    • GSK-3 beta
    • GSK3B
    • GSK3B_HUMAN
    • GSK3beta isoform
    • Serine/threonine-protein kinase GSK3B
    see all
  • Function
    Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair.
  • Tissue specificity
    Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated by AKT1 and ILK1. Activated by phosphorylation at Tyr-216.
  • Cellular localization
    Cytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane.
  • Information by UniProt

Images

  • Sample Kinase Activity Plot.
  • ab60863 on SDS-PAGE, MW ~73 kDa.

  • Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 90 secondsab60863 is a tagged recombinant protein with an expected molecular weight of 73kDa.

References

This product has been referenced in:
  • Suryo Rahmanto A  et al. FBW7 suppression leads to SOX9 stabilization and increased malignancy in medulloblastoma. EMBO J 35:2192-2212 (2016). Read more (PubMed: 27625374) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Functional Studies
Recombinant human GSK3ß (ab60863) was used to phosphorylate putative GSK3ß substrate (protein X). Evidence of phosphorylation was confirmed by:
(1) in-house generated phospho-specific antibody against the putative substrate (Protein X - T236 data shown)
(2) lambda phosphatase treatment of GSK3ß-treated protein X known phospho-specific protein interactor
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Verified customer

Submitted Dec 08 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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