Recombinant human Hck protein (ab60865)

Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • SpeciesHuman
    • Amino acids230 to 497

Associated products

Specifications

Our Abpromise guarantee covers the use of ab60865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • FormLiquid
  • Additional notes

    ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Bmk
    • Hck
    • Hck 1
    • HCK_HUMAN
    • Hemopoietic cell kinase
    • JTK9
    • p59-HCK/p60-HCK
    • p59Hck
    • p59HCK/p60HCK
    • p61Hck
    • Tyrosine protein kinase HCK
    • Tyrosine-protein kinase HCK
    see all
  • FunctionMay serve as part of a signaling pathway coupling the Fc receptor to the activation of the respiratory burst. May also contribute to neutrophil migration and may regulate the degranulation process of neutrophils.
  • Tissue specificityExpressed predominantly in cells of the myeloid and B-lymphoid lineages.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • DomainThe SH3 domain mediates binding to HIV-1 Nef.
  • Cellular localizationMembrane. Cytoplasm. At least half of this isoform is found in the cytoplasm, some of this fraction is myristoylated and Membrane. Membrane > caveola. 20% of this isoform is associated with caveolae, this association depends on palmitoylation of cyteine at position 3.
  • Information by UniProt

Recombinant human Hck protein images

  • Sample Kinase Activity Plot.
  • ab60865 on SDS-PAGE, MW ~57 kDa.

References for Recombinant human Hck protein (ab60865)

ab60865 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. I have received the following information For Wavelength of 280 nm: Molar Extinction w/o Disulfides 81930 M-1 cm-1 Molar Extinction w/ All Disulfides 82290 M-1 cm-1 Molar Extinction w/o D...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"