The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Hydrolysis of N-e-acetyl-lysine residues of histones.
Specific Activity: >2U/µg. One unit is defined as the amount of enzyme that deacetylates 1 pmol/min of Fluor de Lys™-SIRT1 substrate at 37°C / 500µM.
Stable on ice for approx. 1h. Snap freezing in liquid nitrogen is recommended if refreezing of remaining, undiluted enzyme if necessary. Freezing and storage of diluted enzyme is not recommended.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Histone deacetylase 10
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
Ubiquitous. High expression in liver, spleen, pancreas and kidney.
Belongs to the histone deacetylase family. HD type 2 subfamily.