Overview

Description

  • NatureRecombinant
  • SourceBaculovirus
  • Amino Acid Sequence
    • AccessionO15379
    • SpeciesHuman
    • Molecular weight83 kDa including tags
    • Amino acids1 to 428

Specifications

Our Abpromise guarantee covers the use of ab101663 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activitySpecific activity 125 RLU/min/ng.
  • Applications

    SDS-PAGE

    Functional Studies

  • Purity> 70 % SDS-PAGE.
    ab101663 is >70% pure as assessed by densitometry.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • HD3
    • HDAC 3
    • HDAC3
    • HDAC3_HUMAN
    • Histone deacetylase 3
    • RPD3
    • RPD3 2
    • RPD3-2
    • SMAP45
    see all
  • FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
  • Post-translational
    modifications
    Sumoylated in vitro.
  • Cellular localizationNucleus.
  • Information by UniProt

Recombinant human HDAC3 protein images

  • The specific activity of ab101663 was determined to be 125 RLU/min/ng.
  • The purity of HDAC3 was determined to be >70% as assessed by densitometry.
    The approximate molecular weight is ~83 kDa.

References for Recombinant human HDAC3 protein (ab101663)

ab101663 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"