Overview

Description

  • NatureRecombinant
  • SourceBaculovirus
  • Amino Acid Sequence
    • AccessionQ9UQL6
    • SpeciesHuman
    • Molecular weight51 kDa
    • Amino acids656 to 1122
    • TagsHis tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab80351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific Activity: ≥1000 pmol/min/µg.
    Unit Definition: One U =1 pmol of acetyl group removed/min/µg of enzyme.
    Assay Conditions:
    25 mM Tris HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 30 µM HDAC class 2a substrate, and HDAC5. Incubation condition: 30 min at 30°C, followed by HDAC developer for 15 min at room temperature. Fluorescence intensity is measured at ex360/em460.

     

  • Applications

    SDS-PAGE

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 50mM Tris HCl, 138mM Sodium chloride, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Antigen NY CO 9
    • Antigen NY-CO-9
    • HD5
    • HDAC 5
    • HDAC5
    • HDAC5_HUMAN
    • Histone deacetylase 5
    • NY CO 9
    see all
  • FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the histone deacetylase family. HD type 2 subfamily.
  • DomainThe nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
  • Post-translational
    modifications
    Phosphorylated by CaMK at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
    Ubiquitinated. Polyubiquitination however does not lead to its degradation.
  • Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by CaMK.
  • Information by UniProt

References for Recombinant human HDAC5 protein (ab80351)

ab80351 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"