Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • SpeciesHuman

Specifications

Our Abpromise guarantee covers the use of ab42633 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity10 U/ug. One U =1 pmol/min, Assay condition: 25 mM Tris/Cl, pH8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 100 uM Biomol substrate (Catalog number KI-178), and 15 ng/ul HDAC8. Incubation condition: 60 min at 30ºC.
  • Applications

    Inhibition Assay

  • Purity> 90 % SDS-PAGE.

  • FormLiquid
  • Additional notesExpressed in a Baculovirus infected Sf9 cell expression system. MW= 46.4 kDa. 10 U/ug. One U =1 pmol/min, Assay condition: 25 mM Tris/Cl, pH8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 100 uM Biomol substrate (Catalog number KI-178), and 15 ng/ul HDAC8. Incubation condition: 60 min at 30ºC.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 50mM Tris HCl, 138mM Sodium chloride, pH 8

General Info

  • Alternative names
    • CDA07
    • CDLS5
    • HD 8
    • HD8
    • HDAC 8
    • Hdac8
    • HDAC8_HUMAN
    • HDACL 1
    • HDACL1
    • Histone deacetylase 8
    • Histone deacetylase like 1
    • MRXS6
    • RPD 3
    • RPD3
    • WTS
    see all
  • FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility.
  • Tissue specificityWeakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney.
  • Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
  • Post-translational
    modifications
    Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4.
  • Cellular localizationNucleus. Cytoplasm. Excluded from the nucleoli. Found in the cytoplasm of cells showing smooth muscle differentiation.
  • Information by UniProt

References for Recombinant Human HDAC8 protein (ab42633)

ab42633 has not yet been referenced specifically in any publications.

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