- Datasheet
- Specific References
- Protocols
Overview
- Product nameRecombinant Human HDAC8 protein
See all HDAC8 proteins and peptides - Protein lengthFull length protein
Description
- NatureRecombinant
- SourceBaculovirus infected Sf9 cells
- Amino Acid Sequence
- SpeciesHuman
Associated products
- Related Products
Specifications
Our Abpromise guarantee covers the use of ab42633 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- Biological activity10 U/ug. One U =1 pmol/min, Assay condition: 25 mM Tris/Cl, pH8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 100 uM Biomol substrate (Catalog number KI-178), and 15 ng/ul HDAC8. Incubation condition: 60 min at 30ºC.
- Applications
Inhibition Assay
- Purity> 90
% SDS-PAGE.
- FormLiquid
- Additional notesExpressed in a Baculovirus infected Sf9 cell expression system. MW= 46.4 kDa. 10 U/ug. One U =1 pmol/min, Assay condition: 25 mM Tris/Cl, pH8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 100 uM Biomol substrate (Catalog number KI-178), and 15 ng/ul HDAC8. Incubation condition: 60 min at 30ºC.
-
Concentration information loading...
Preparation and Storage
- Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Preservative: None
Constituents: 10% Glycerol, 50mM Tris HCl, 138mM Sodium chloride, pH 8
General Info
-
Alternative names
- CDA07
- CDLS5
- HD 8
see all - FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility.
- Tissue specificityWeakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney.
- Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
- Post-translational
modificationsPhosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4. - Cellular localizationNucleus. Cytoplasm. Excluded from the nucleoli. Found in the cytoplasm of cells showing smooth muscle differentiation.
- Information by UniProt
References for Recombinant Human HDAC8 protein (ab42633)
ab42633 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"