The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
>90% by SDS-PAGE.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.0 Constituents: 0.32% Tris HCl, 2.4% Urea, 10% Glycerol
Cyclin E binding protein 1
Cyclin-E-binding protein 1
E3 ISG15--protein ligase HERC5
HECT domain and RCC1 like domain containing protein 5
HECT domain and RCC1-like domain-containing protein 5
Hect domain and RLD 5
HECT E3 ubiquitin ligase
Major E3 ligase for ISG15 conjugation. Acts as a positive regulator of innate antiviral response in cells induced by interferon. Makes part of the ISGylation machinery that recognizes target proteins in a broad and relatively non-specific manner. Catalyzes ISGylation of IRF3 which results in sustained activation, it attenuates IRF3-PIN1 interaction, which antagonizes IRF3 ubiquitination and degradation, and boosts the antiviral response. Catalyzes ISGylation of influenza A viral NS1 which attenuates virulence; ISGylated NS1 fails to form homodimers and thus to interact with its RNA targets. Catalyzes ISGylation of papillomavirus type 16 L1 protein which results in dominant-negative effect on virus infectivity. Physically associated with polyribosomes, broadly modifies newly synthesized proteins in a cotranslational manner. In an interferon-stimulated cell, newly translated viral proteins are primary targets of ISG15.
Expressed in testis and to a lesser degree in brain, ovary and placenta. Found in most tissues at low levels.