Recombinant Human Heme Oxygenase 1 protein (ab85243)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, WB
Description
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Product name
Recombinant Human Heme Oxygenase 1 protein
See all Heme Oxygenase 1 proteins and peptides -
Purity
> 95 % SDS-PAGE.
Purity was determined to be >95% by densitometry. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MERPQPDSMPQDLSEALKEATKEVHTQAENAEFMRNFQKGQVTRDGFKLV MASLYHIYVALEEEIERNKESPVFAPVYFPEELHRKAALEQDLAFWYGPR WQEVIPYTPAMQRYVKRLHEVGRTEPELLVAHAYTRYLGDLSGGQVLKKI AQKALDLPSSGEGLAFFTFPNIASATKFKQLYRSRMNSLEMTPAVRQRVI EEAKTAFLLNIQLFEELQELLTHDTKDQSPSRAPGLRQRASNKVQDSAPV ETPRGKPPLNTRSQAPLLRWVLTLSFLVATVAVGLYAM -
Predicted molecular weight
56 kDa including tags -
Amino acids
1 to 288 -
Tags
GST tag N-Terminus
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Description
Recombinant Human Heme Oxygenase 1 protein (Tagged)
Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab85243 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Western blot
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
General Info
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Alternative names
- 32 kD
- bK286B10
- D8Wsu38e
see all -
Function
Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. -
Sequence similarities
Belongs to the heme oxygenase family. -
Cellular localization
Microsome. Endoplasmic reticulum. - Information by UniProt
Images
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SDS-PAGE showing ab85243 at approximately 56kDa.
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Standard Curve for Heme Oxygenase 1 (Analyte: Heme Oxygenase 1 protein (Tagged) (ab85243)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [HO-1-1] to Heme Oxygenase 1 (ab13248) at 5µg/ml and Detector Antibody Rabbit polyclonal to Heme Oxygenase 1 (ab13243) at 0.5µg/ml.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab85243 has not yet been referenced specifically in any publications.