Recombinant human Hexokinase 1 protein (ab85918)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceMGSSHHHHHH SSGLVPRGSH MIAAQLLAYY FTELKDDQVK KIDKYLYAMR LSDETLIDIM TRFRKEMKNG LSRDFNPTAT VKMLPTFVRS IPDGSEKGDF IALDLGGSSF RILRVQVNHE KNQNVHMESE VYDTPENIVH GSGSQLFDHV AECLGDFMEK RKIKDKKLPV GFTFSFPCQQ SKIDEAILIT WTKRFKASGV EGADVVKLLN KAIKKRGDYD ANIVAVVNDT VGTMMTCGYD DQHCEVGLII GTGTNACYME ELRHIDLVEG DEGRMCINTE WGAFGDDGSL EDIRTEFDRE IDRGSLNPGK QLFEKMVSGM YLGELVRLIL VKMAKEGLLF EGRITPELLT RGKFNTSDVS AIEKNKEGLH NAKEILTRLG VEPSDDDCVS VQHVCTIVSF RSANLVAATL GAILNRLRDN KGTPRLRTTV GVDGSLYKTH PQYSRRFHKT LRRLVPDSDV RFLLSESGSG KGAAMVTAVA YRLAEQHRQI EETLAHFHLT KDMLLEVKKR MRAEMELGLR KQTHNNAVVK MLPSFVRRTP DGTENGDFLA LDLGGTNFRV LLVKIRSGKK RTVEMHNKIY AIPIEIMQGT GEELFDHIVS CISDFLDYMG IKGPRMPLGF TFSFPCQQTS LDAGILITWT KGFKATDCVG HDVVTLLRDA IKRREEFDLD VVAVVNDTVG TMMTCAYEEP TCEVGLIVGT GSNACYMEEM KNVEMVEGDQ GQMCINMEWG AFGDNGCLDD IRTHYDRLVD EYSLNAGKQR YEKMISGMYL GEIVRNILID FTKKGFLFRG QISETLKTRG IFETKFLSQI ESDRLALLQV RAILQQLGLN STCDDSILVK TVCGVVSRRA AQLCGAGMAA VVDKIRENRG LDRLNVTVGV DGTLYKLHPH FSRIMHQTVK ELSPKCNVSF LLSEDGSGKG AALITAVGVR LRTEASS
    • Amino acids1 to 917
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab85918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activitySpecific activity is >2 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. In the coupled mode, one unit will produce 1.0 umole of NADPH per minute as glucose is phosphorylated by ATP at pH 7.4 at 25C.
  • Applications

    Functional Studies

    SDS-PAGE

    Western blot

  • Purity> 90 % SDS-PAGE.
    ab85918 is purified using conventional chromatography techniques.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • BB404130
    • Brain form hexokinase
    • dea
    • DrHXK1
    • EC 2.7.1.1
    • Glycolytic enzyme
    • HEXOKIN
    • hexokinase I
    • Hexokinase PI
    • Hexokinase type I
    • Hexokinase, tumor isozyme
    • Hexokinase-1
    • Hexokinase-A
    • HK I
    • HK1
    • HK1 tb
    • Hk1-s
    • HK1-ta
    • HK1-tb
    • HK1-tc
    • HKD
    • HKI
    • HMSNR
    • HXK1
    • HXK1_HUMAN
    • im:7148527
    • mHk1-s
    • wu:fc09d08
    • wu:fc16e02
    • wu:fc21e02
    • wu:fq14b11
    • zgc:55790
    • zgc:77618
    see all
  • Tissue specificityIsoform 2 is erythrocyte specific. Isoform 3 and isoform 4 are testis-specific.
  • PathwayCarbohydrate metabolism; hexose metabolism.
  • Involvement in diseaseHexokinase deficiency
    Neuropathy, hereditary motor and sensory, Russe type
  • Sequence similaritiesBelongs to the hexokinase family.
    Contains 2 hexokinase domains.
  • DomainThe N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule.
  • Cellular localizationMitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding.
  • Information by UniProt

Recombinant human Hexokinase 1 protein images

  • 12% SDS-PAGE showing ab85918 at approximately 104.6kDa.
  • Anti-Hexokinase 1 antibody (ab65069) at 1/500 dilution + Recombinant human Hexokinase 1 protein (ab85918) at 0.1 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 4 minutes

References for Recombinant human Hexokinase 1 protein (ab85918)

ab85918 has not yet been referenced specifically in any publications.

Product Wall

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Q1. I was wondering if the specific activity calculated for the his-tagged Hexokinase type-1 protein (ab85918) includes the coupling effects of oxidative phosphorylation might have, as a source of ATP?

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Thank you for your inquiry. There is only one His-tag, and it's located at the N-terminus. I hope this information helps. Please contact us with any other questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"