The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Specific activity is 3-4 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. In the coupled mode, one unit will produce 1.0 umole of NADPH per minute as glucose is phosphorylated by ATP at pH 7.4 at 30C.
% SDS-PAGE. ab85920 is prified using conventional chromatography techniques.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 10% Glycerol, 20mM Tris HCl, pH 8.0
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Hexokinase 2 muscle
Hexokinase type II
Muscle form hexokinase
Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle.
Carbohydrate metabolism; hexose metabolism.
Belongs to the hexokinase family. Contains 2 hexokinase domains.
The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule.
Mitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding.