Overview

Description

  • NatureRecombinant
  • SourceCHO cells
  • Amino Acid Sequence
    • AccessionP14210
    • SpeciesHuman
    • Molecular weight80 kDa
    • Amino acids32 to 728

Specifications

Our Abpromise guarantee covers the use of ab105061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityThe ED50, calculated by the dose-dependent proliferation of monkey 4MBr-5 indicator cells was found to be 20-40 ng/ml.
  • Applications

    SDS-PAGE

    Functional Studies

    Western blot

  • Purity> 95 % SDS-PAGE.
    Purified by proprietary chromatographic techniques. Purity is >97% by SDS-PAGE and HPLC analyses
  • FormLyophilised
  • Additional notesUpon reconstitution ab105061 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.

    Preservative: None
    Constituents: 0.05% Tween 20, PBS, 100mM L-arginine, pH 7.4

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionReconstitute in sterile 18Mohm-cm-¹ H2O at not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

General Info

  • Alternative names
    • DFNB39
    • F TCF
    • Fibroblast derived tumor cytotoxic factor
    • Hepatocyte growth factor
    • Hepatocyte growth factor (hepapoietin A; scatter factor)
    • Hepatocyte growth factor beta chain
    • Hepatocyte growth factor precursor
    • Hepatopoietin A
    • Hepatopoietin-A
    • Hgf
    • HGF_HUMAN
    • HGFB
    • HPTA
    • Lung fibroblast derived mitogen
    • OTTHUMP00000161349
    • OTTHUMP00000206710
    • OTTHUMP00000206711
    • OTTHUMP00000206712
    • OTTHUMP00000206713
    • OTTHUMP00000206730
    • Scatter factor
    • SF
    see all
  • FunctionPotent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.
  • Involvement in diseaseDeafness, autosomal recessive, 39
  • Sequence similaritiesBelongs to the peptidase S1 family. Plasminogen subfamily.
    Contains 4 kringle domains.
    Contains 1 PAN domain.
    Contains 1 peptidase S1 domain.
  • Information by UniProt

Recombinant human HGF protein images

  • Anti-HGF antibody (ab24865) at 1 µg/ml + Recombinant human HGF protein (ab105061) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

References for Recombinant human HGF protein (ab105061)

ab105061 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"