Recombinant human HGF protein (ab632)

Overview

Description

  • NatureRecombinant
  • SourceBTI-TN-5B1-4 cells
  • Amino Acid Sequence
    • SpeciesHuman
    • SequencehHGF (Human Hepatocyte Growth Factor) is an 80 kDa disulfide-linked heterodimeric protein consisting of the alpha chain (463 amino acids) and the beta chain (234 amino acids): QRKRRNTIHE FKKSAKTTLI KIDPALKIKT KKVNTA DQCA NRCTRNKGLP FTCKAFVFDKARKQCLWFPF NSMSSQVKKE FG HEFDLYEN KDYIRNCIIG KGRSYKGTVS ITKSGIKCQPWSSMIPHEH S FLPSSYRGKD LQENYCRNPR GEEGGPWCFT SNPEVRYEVC DIPQ CSEVECMTCNGESYRG LMGHTESGKI CQRWDHQTPH RHKFLPERYP DKGFDDNYCR NPDGGPRPWCYTLDPHTRWE YCAIKTCADN TMNDTDV PLE TTECIQGQGE GYRGTVNTIW NGIPCQRWDSQYPHEHDMTP ENF KCKDLRE NYCRNPDGSE SPWCFTTDPN IRVGYCSQIP NCDMSHGQD CYRGNGKNYMG NLSQTRSGLT CSMWDKNMED LHRHIFWEPD ASKLN ENYCR NPDDDAHGPWCYTGNPLIPW DYCPISRCEG DTTPTIVNLD H PVISCAKTK QLRVVNGIPT RTNIGWMVSLRYRNKHICGG SLIKESWV LT ARQCFPSRDL KDYEAWLGIH DVHGRGDEKC KQVLNVSQLVYGPE GSDLVL MKLARPAVLD DFVSTIDLPN YGCTIPEKTS CSVYGWGYTG LINYDGLLRVAHLYIMGNEK CSQHHRGKVT LNESEICAGA EKIGSQ PCEG DYGGPLVCEQ HKMRMVLGVIVPGRGCAIPN RPGIFVRVAY YA KWIHKIIL TYKVPQS

Specifications

Our Abpromise guarantee covers the use of ab632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • FormLyophilised
  • Additional notesThe ED50, determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells, was found to be in the range of 20.0 - 40.0 ng/ml. Expression System = Baculovirus infected High-5 cells.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.0
    Constituents: 1.16% Sodium chloride, 0.12% Tris, 1.74% Arginine

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionCentrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use.

General Info

  • Alternative names
    • DFNB39
    • F TCF
    • Fibroblast derived tumor cytotoxic factor
    • Hepatocyte growth factor
    • Hepatocyte growth factor (hepapoietin A; scatter factor)
    • Hepatocyte growth factor beta chain
    • Hepatocyte growth factor precursor
    • Hepatopoietin A
    • Hepatopoietin-A
    • Hgf
    • HGF_HUMAN
    • HGFB
    • HPTA
    • Lung fibroblast derived mitogen
    • OTTHUMP00000161349
    • OTTHUMP00000206710
    • OTTHUMP00000206711
    • OTTHUMP00000206712
    • OTTHUMP00000206713
    • OTTHUMP00000206730
    • Scatter factor
    • SF
    see all
  • FunctionPotent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.
  • Involvement in diseaseDeafness, autosomal recessive, 39
  • Sequence similaritiesBelongs to the peptidase S1 family. Plasminogen subfamily.
    Contains 4 kringle domains.
    Contains 1 PAN domain.
    Contains 1 peptidase S1 domain.
  • Information by UniProt

Recombinant human HGF protein images

  • Standard curve for HGF (Analyte: ab632); dilution range 1pg/ml to 1µg/ml using Capture Antibody ab10829 at 5µg/ml and Detector Antibody ab24865 at 0.5µg/ml.

References for Recombinant human HGF protein (ab632)

ab632 has not yet been referenced specifically in any publications.

Product Wall

I can confirm the product ab632 human HGF is supplied as the active (mature form). Please contact us again if you need further information,

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"