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Our Abpromise guarantee covers the use of ab154478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Product was previously marketed under the MitoSciences sub-brand.
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.61% Tris, 10% Glycerol, 0.88% Sodium chloride
1µg of ab154478 was examined by SDS-PAGE and commassie blue protein stain. Full-length HIF-1-alpha is indicated by the arrow. Purity is judged to be >75%.
Expected MW is 100kDa.
ab154478 was examined by western blot with an anti-HIF-1-alpha antibody.
Lane1: 10ng HIF1 alpha full-length protein (ab154478)
Block: 4% milk/PBS Primary antibody: anti-HIF-1-alpha (ab51608), 1:1000; 2 hours room temperature. Secondary antibody: anti-Rabbit HRP 1:5000 ECL detection
ab154478 has not yet been referenced specifically in any publications.