Recombinant Human HINT1 protein (ab87362)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceMADEIAKAQV ARPGGDTIFG KIIRKEIPAK IIFEDDRCLA FHDISPQAPT HFLVIPKKHI SQISVAEDDD ESLLGHLMIV GKKCAADLGL NKGYRMVVNE GSDGGQSVYH VHLHVLGGRQ MHWPPG

Specifications

Our Abpromise guarantee covers the use of ab87362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity> 90 % SDS-PAGE.
    Purified by using conventional chromatography.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, pH 8

General Info

  • Alternative names
    • Adenosine 5' monophosphoramidase
    • Adenosine 5''-monophosphoramidase
    • HINT 1
    • HINT1
    • HINT1_HUMAN
    • Histidine triad nucleotide binding protein 1
    • Histidine triad nucleotide-binding protein 1
    • PKCI 1
    • PKCI-1
    • PKCI1
    • PRKCNH1
    • Protein kinase C inhibitor 1
    • Protein kinase C interacting protein 1
    • Protein kinase C-interacting protein 1
    see all
  • FunctionHydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the HINT family.
    Contains 1 HIT domain.
  • DomainThe histidine triad, also called HIT motif, forms part of the binding loop for the alpha-phosphate of purine mononucleotide.
  • Cellular localizationCytoplasm. Nucleus. Interaction with CDK7 leads to a more nuclear localization.
  • Information by UniProt

Recombinant Human HINT1 protein images

  • ab87362 on 15% SDS-PAGE (3µg)

References for Recombinant Human HINT1 protein (ab87362)

ab87362 has not yet been referenced specifically in any publications.

Product Wall

Application Other

Review text: We have tested enzymatic activity of the HINT1 protein (ab87362)
This enzyme hydrolyzes adenosine 5'-phosphoramidate (H2NPrA) to adenosine 5'-phosphate (rAMP).
The protocol which we used in the assay.

Fig1.
Enzymatic reaction was carried out under the following conditions:
Solution of substrate (adenosine 5'-phosphoramidate) at 1 mM were prepared in 20 mM Na-HEPES (pH 7.2) containing 0.5 mM of MgCl2 and the HINT1 (ab87362, 0.5, 1.0 or 2.0 µg) in 25 µl total volume.
Through 15, 30, 60, 120 min and 18 h aliquots were taken (1 µl each). Aliquots were analyzed on TLC-plates in Silica gel 60 F254 (Merck) in system dioxan-isopropanol-25% aqueous ammonia-water – 8:7:3:2.
As can be seen from the figure, we can assume:
The hydrolysis of 50% amount of H2NPrA was 5 min with 2 µg of HINT1 2 mg/ml for 30 min with 1 µg of HINT1 and more than 60 min with 0.5 µg of HINT1.

Fig2.
Enzymatic reaction was carried out under the following conditions:
Solution of substrate (adenosine 5'-phosphoramidate) at 1 mM were prepared in 20 mM Na-HEPES (pH 7.2) containing 0, 0.2 or 0.5 mM of MgCl2 and the HINT1 (ab87362, 0.5 or 1.0 µg) in 25 µl total volume.
Through 15, 30, 60, 120 and 180 min aliquots were taken (1 µl each). Aliquots were analyzed on TLC-plates in Silica gel 60 F254 (Merck) in system dioxan-isopropanol-25% aqueous ammonia-water – 8:7:3:2.
As can be seen from the figure, we can assume:
HINT1 - magnesium-independent enzyme.
The hydrolysis of 50% amount of H2NPrA was for 15-30 min with 1 µg of HINT1 and less than 120 min with 0.5 µg HINT1.

When using HINT1 (ab87362) substrate hydrolysis was observed only up to adenosine 5’-monophosphate. During the enzymatic hydrolysis was not observed the formation of adenosine. In the absence of the enzyme in the reaction conditions adenosine 5'-phosphoramidate was stable.
Sample: Human Recombinant protein

Primary antibody (in addition to 'HINT1 protein (Human)')
Primary antibody: None used

Secondary antibody
Secondary antibody: None used
Username

Mr. Alexander Shipitsyn

Verified customer

Submitted Nov 19 2012

Thank you for your email.

I am very pleased to hear you would like to accept our offer and test ab87362 for its biological acitivty. The code below will give you the equivalent price of ab87362 off any product in our catalogue.To redeem this ...

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Sorry for the delay in getting back to you.I now have some further information to share with you.

Regrettably, theHINT1 protein (ab87362)biological activity has as yet not beentested. We do not therefore know if it has retained the enzymatic ...

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Thank you for contacting us and your interest in our products.

I am sorry but I do not think I completely understand your question. The HINT1 protein (Human) (ab87362) is the full length recombinant human protein expressed in E. coli. The pro...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"