Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionP05114
    • SpeciesHuman
    • SequenceMPKRKVSSAE GAAKEEPKRR SARLSAKPPA KVEAKPKKAA AKDKSSDKKV QTKGKRGAKG KQAEVANQET KEDLPAENGE TKTEESPASD EAGEKEAKSD LEHHHHHH
    • Molecular weight12 kDa including tags
    • Amino acids1 to 100
    • TagsHis tag C-Terminus
    • Additional sequence informationHMGN1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 108 amino acids (1-100 a.a.) and having a molecular mass of 11.7 kDa.

Specifications

Our Abpromise guarantee covers the use of ab117159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    ab117159 was purified by standard chromatography and filter sterilized.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.32% Tris HCl, 0.02% DTT, 0.58% Sodium chloride, 0.003% PMSF, 20% Glycerol

General Info

  • Alternative names
    • FLJ27265
    • FLJ31471
    • High mobility group (nonhistone chromosomal) protein 14
    • High mobility group nucleosome binding 1
    • High mobility group nucleosome binding domain 1
    • High mobility group nucleosome binding domain containing protein 1
    • High mobility group nucleosome-binding domain-containing protein 1
    • High mobility group protein 14
    • HMG 14
    • HMG14
    • HMGN 1
    • HMGN1
    • HMGN1_HUMAN
    • MGC104230
    • MGC117425
    • Non-histone chromosomal protein HMG-14
    • Nonhistone chromosomal protein HMG 14
    • Nonhistone chromosomal protein HMG14
    see all
  • FunctionBinds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in an unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2.
  • Sequence similaritiesBelongs to the HMGN family.
  • Post-translational
    modifications
    Phosphorylation on Ser-21 and Ser-25 weakens binding to nucleosomes and increases the rate of H3 phosphorylation (By similarity). Phosphorylation favors cytoplasmic localization.
  • Cellular localizationNucleus. Cytoplasm. Cytoplasmic enrichment upon phosphorylation. The RNA edited version localizes to the nucleus.
  • Information by UniProt

References for Recombinant Human HMG14 protein (ab117159)

ab117159 has not yet been referenced specifically in any publications.

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