Overview

  • Product nameRecombinant Human HNF6 protein
  • Protein lengthFull length protein

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionQ9UBC0
    • SpeciesHuman
    • Molecular weight51 kDa
    • Amino acids1 to 465

Specifications

Our Abpromise guarantee covers the use of ab98300 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

    Dot blot

    SDS-PAGE

  • Purity> 90 % SDS-PAGE.
    ab98300 was expressed in E. coli and refolded to soluble form.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 500 mM L-arginine, 300 mM guanidine-HCl, 50 mM HEPES, 5 mM cystamine, 5 mM cysteamine, 5 mM Calcium chloride, 3 mM DTT, pH 7.5

General Info

  • Alternative names
    • Hepatocyte nuclear factor 6
    • hepatocyte nuclear factor 6 alpha
    • HNF 6
    • HNF-6
    • HNF6_HUMAN
    • HNF6A
    • One cut domain family member 1
    • One cut homeobox 1
    • Onecut1
    see all
  • FunctionTranscriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. Important for liver genes transcription.
  • Tissue specificityHighly expressed in liver; lower expression in testis and skin.
  • Sequence similaritiesBelongs to the CUT homeobox family.
    Contains 1 CUT DNA-binding domain.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt

Recombinant Human HNF6 protein images

  • HNF6 monoclonal antibody at 1 µg/ml + Recombinant Human HNF6 protein (ab98300) at 0.05 µg
  • SDS-PAGE analysis of 1.7µg ab98300 stained with Coomassie dye.
  • Specific detection of ab98300 (non-denatured) printed in duplicate on Spots On Dots™ nitrocellulose membrane using 1 µg/ml HNF6 monoclonal antibody

References for Recombinant Human HNF6 protein (ab98300)

ab98300 has not yet been referenced specifically in any publications.

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