Recombinant Human hnRNP A1 (isoform A1-A) protein (ab91691)

Overview

  • Product nameRecombinant Human hnRNP A1 (isoform A1-A) protein
  • Protein lengthFull length protein

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceMSKSESPKEPEQLRKLFIGGLSFETTDESLRSHFEQWGTLTDCVVMRDPN TKR SRGFGFVTYATVEEVDAAMNARPHKVDGRVVEPKRAVSREDSQRP GAHLTVKK IFVGGIKEDTEEHHLRDYFEQYGKIEVIEIMTDRGSGKKR GFAFVTFDDHDSV DKIVIQKYHTVNGHNCEVRKALSKQEMASASSSQR GRSGSGNFGGGRGGGFGG NDNFGRGGNFSGRGGFGGSRGGGGYGGSGD GYNGFGNDGSNFGGGGSYNDFGN YNNQSSNFGPMKGGNFGGRSSGPYG GGGQYFAKPRNQGGYGGSSSSSSYGSGR RF (Amino acid sequence (Sequence determined by 5' Sequencing))

Specifications

Our Abpromise guarantee covers the use of ab91691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry

    SDS-PAGE

    Western blot

  • FormLyophilised
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS)(acquired on initial reference batch)
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium hydrogen phosphate, 10mM Sodium chloride, pH 4.5

  • ReconstitutionReconstitute with 50 µl distilled water.

General Info

  • Alternative names
    • Helix-destabilizing protein
    • Heterogeneous nuclear ribonucleoprotein A1
    • HN RPA1L3
    • hnRNP A1
    • hnRNP core protein A1
    • HNRNPA1
    • M MGC102835
    • ROA1_HUMAN
    • Single-strand RNA-binding protein
    see all
  • FunctionInvolved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. May play a role in HCV RNA replication.
  • Sequence similaritiesContains 2 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications
    Arg-194, Arg-206 and Arg-225 are dimethylated, probably to asymmetric dimethylarginine.
    Sumoylated.
  • Cellular localizationNucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles continuously between the nucleus and the cytoplasm along with mRNA. Component of ribonucleosomes. In the course of viral infection, colocalizes with HCV NS5B at speckles in the cytoplasm in a HCV-replication dependent manner.
  • Information by UniProt

Recombinant Human hnRNP A1 (isoform A1-A) protein images

  • The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

References for Recombinant Human hnRNP A1 (isoform A1-A) protein (ab91691)

ab91691 has not yet been referenced specifically in any publications.

Product Wall

Thanks you for your enquiry. No enzymatic activity was tested for ab91691 yet. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"