Recombinant Human hnRNP C1 + C2 protein (ab125177)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionP07910
    • SpeciesHuman
    • SequenceMGSSHHHHHH SSGLVPRGSH MASNVTNKTD PRSMNSRVFI GNLNTLVVKK SDVEAIFSKY GKIVGCSVHK GFAFVQYVNE RNARAAVAGE DGRMIAGQVL DINLAAEPKV NRGKAGVKRS AAEMYGSSFD LDYDFQRDYY DRMYSYPARV PPPPPIARAV VPSKRQRVSG NTSRRGKSGF NSKSGQRGSS KSGKLKGDDL QAIKKELTQI KQKVDSLLEN LEKIEKEQSK QAVEMKNDKS EEEQSSSSVK KDETNVKMES EGGADDSAEE GDLLDDDDNE DRGDDQLELI KDDEKEAEEG EDDRDSANGE DDS
    • Molecular weight35 kDa including tags
    • Amino acids1 to 293
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab125177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity> 85 % SDS-PAGE.
    ab125177 is purified by proprietary chromatographic techniques. Purity is greater than 85.0% as determined by SDS-PAGE.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.08% DTT, 0.32% Tris HCl, 30% Glycerol, 1.17% Sodium chloride

General Info

  • Alternative names
    • C1
    • C2
    • Heterogeneous nuclear ribonucleoprotein C
    • Heterogeneous nuclear ribonucleoprotein C (C1/C2)
    • Heterogeneous nuclear ribonucleoproteins C1/C2
    • HNRNP
    • hnRNP C1 / hnRNP C2
    • hnRNP C1/C2
    • Hnrnpc
    • HNRPC
    • HNRPC_HUMAN
    • MGC104306
    • MGC105117
    • MGC117353
    • MGC131677
    • Nuclear ribonucleoprotein particle C1 protein
    • Nuclear ribonucleoprotein particle C2 protein
    • SNRPC
    see all
  • FunctionBinds pre-mRNA and nucleates the assembly of 40S hnRNP particles (PubMed:8264621). Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, a modification present at internal sites of mRNAs that affects mRNA splicing, processing and stability. M6A alters the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch' to facilitate binding of HNRNPC, leading to regulation of mRNA splicing (PubMed:25719671). Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules (PubMed:12509468, PubMed:16010978, PubMed:7567451, PubMed:8264621).
  • Sequence similaritiesBelongs to the RRM HNRPC family. RALY subfamily.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications
    Phosphorylated on Ser-260 and Ser-299 in resting cells. Phosphorylated on Ser-253 and on 1 serine residue in the poly-Ser stretch at position 238 in response to hydrogen peroxide.
    Sumoylated. Sumoylation reduces affinity for mRNA.
  • Cellular localizationNucleus. Component of ribonucleosomes.
  • Information by UniProt

References for Recombinant Human hnRNP C1 + C2 protein (ab125177)

ab125177 has not yet been referenced specifically in any publications.

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