Recombinant Human hnRNP Q protein (ab153090)

Overview

Description

  • Nature
    Recombinant
  • Source
    Wheat germ
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      MEDHLQIPFIQIFVGKIPRDLFEDELVPLFEKAGPIWDLRLMMDPLTGLN RGYAFVTFCTKEAAQEAVKLYNNHEIRSGKHIGVCISVANNRLFVGSIPK SKTKEQILEEFSKVTEGLTDVILYHQPDDKKKNRGSCFLEYEDHKTAAQA RRRLMSGKVKVWGNVGTVEWADPIEDPDPEVMAKVKVLFVRNLANTVTEE ILEKAFSQFGKLERVKKLKDYAFIHFDERDGAVKAMEEMNGKDLEGENIE IVFAKPPDQKRKERKAQRQAAKNQMYDDYYYYGPPHMPPPTRGRGRGGRG GYGYPPDYYGYEDYYDYYGYDYHNYRGGYEDPYYGYEDFQVGARGRGGRG ARGAAPSRGRGAAPPRGRAGYSQRGGPGSARGVRGARGGAQQQRGRGQGK GVEAGPDLLQ
    • Amino acids
      1 to 410
    • Tags
      proprietary tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab153090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    Western blot

  • Form
    Liquid
  • Additional notes
    Protein concentration is above or equal to 0.05 mg/ml.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.31% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names
    • cytoplasmic RNA-interacting protein
    • dJ3J17.2
    • Glycine and tyrosine rich RNA binding protein
    • Glycine- and tyrosine-rich RNA-binding protein
    • GRY RBP
    • GRY-RBP
    • GRYRBP
    • Heterogeneous nuclear ribonucleoprotein Q
    • hnRNP Q
    • HNRPQ
    • HNRPQ_HUMAN
    • HNRPQ1
    • NS1 associated protein 1
    • NS1-associated protein 1
    • NSAP1
    • pp68
    • RP1 3J17.2
    • Synaptotagmin binding cytoplasmic RNA interacting protein
    • Synaptotagmin-binding
    • Syncrip
    see all
  • Function
    Heterogenous nuclear ribonucleoprotein (hnRNP) implicated in mRNA processing mechanisms. Component of the CRD-mediated complex that promotes MYC mRNA stability. Isoform 1, isoform 2 and isoform 3 are associated in vitro with pre-mRNA, splicing intermediates and mature mRNA protein complexes. Isoform 1 binds to apoB mRNA AU-rich sequences. Isoform 1 is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to A1CF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Interacts in vitro preferentially with poly(A) and poly(U) RNA sequences. Isoform 3 may be involved in cytoplasmic vesicle-based mRNA transport through interaction with synaptotagmins.
  • Tissue specificity
    Ubiquitously expressed. Detected in heart, brain, pancreas, placenta, spleen, lung, liver, skeletal muscle, kidney, thymus, prostate, uterus, small intestine, colon, peripheral blood and testis.
  • Sequence similarities
    Contains 3 RRM (RNA recognition motif) domains.
  • Domain
    The domain containing eight Arg-Gly-Gly repeats may be involved in RNA-binding and protein-protein interactions.
  • Post-translational
    modifications
    Phosphorylated on tyrosine. The membrane-bound form found in microsomes is phosphorylated in vitro by insulin receptor tyrosine kinase (INSR). Phosphorylation is inhibited upon binding to RNA, whereas the cytoplasmic form is poorly phosphorylated (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus > nucleoplasm. Expressed predominantly in the nucleoplasm and Cytoplasm. Microsome. Endoplasmic reticulum. Nucleus. The tyrosine phosphorylated form bound to RNA is found in microsomes (By similarity). Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt

Images

  • ab153090 on a 12.5% SDS-PAGE stained with Coomassie Blue.

References

ab153090 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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