The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% SDS-PAGE. Please filter the product by an appropriate sterile filter before using it in the cell culture.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
0.05 M MES buffer pH 6.5
Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. This product typically requires 20min at 37°C for complete reconstitution. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10µg/ml. In higher concentrations the solubility of this antigen is limited.
Human Prostate Specific Gene 1
Prostate Specific Gene 1
HPG1 (Human Prostate-Specific Gene-1) is a recently discovered membrane-anchored protein of approximately 15 kD. It was detected exclusively in the prostate (19 different tissues were tested). Antisense-oligonicleotide inhibition of HPG1 expression inhibited the growth of one prostate cancer cell line (LNCaP) significantly (86%). HPG1 might be a novel diagnostic and/or therapeutic target.
SDS-PAGE - HPG1 protein (His tag) (ab63279)
LANE 1. M.W. marker - 14, 21, 31, 45, 66, 97 kDa. LANE 2. reduced and heated sample, 5µg/lane. LANE 3. non-reduced and non-heated sample, 5µg/lane.
has not yet been referenced specifically in any publications.
Publishing research using ab63279? Please let us know so that we can cite the reference in this datasheet.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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