Recombinant Human HS3ST1 protein (ab171694)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MGSSHHHHHH SSGLVPRGSH MGSRPAELGQ QELLRKAGTL QDDVRDGVAP NGSAQQLPQT IIIGVRKGGT RALLEMLSLH PDVAAAENEV HFFDWEEHYS HGLGWYLSQM PFSWPHQLTV EKTPAYFTSP KVPERVYSMN PSIRLLLILR DPSERVLSDY TQVFYNHMQK HKPYPSIEEF LVRDGRLNVD YKALNRSLYH VHMQNWLRFF PLRHIHIVDG DRLIRDPFPE IQKVERFLKL SPQINASNFY FNKTKGFYCL RDSGRDRCLH ESKGRAHPQV DPKLLNKLHE YFHEPNKKFF ELVGRTFDWH
    • Molecular weight
      36 kDa including tags
    • Amino acids
      21 to 307
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab171694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity
    >90% by SDS-PAGE.
    ab171694 is purified using conventional chromatography techniques
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.03% DTT, 0.32% Tris HCl, 40% Glycerol, 1.17% Sodium chloride

General Info

  • Alternative names
    • 3-OST-1
    • 3OST
    • 3OST1
    • EC 2.8.2.23
    • h3 OST 1
    • h3-OST-1
    • Heparan sulfate (glucosamine) 3 O sulfotransferase 1
    • Heparan sulfate 3 O sulfotransferase 1
    • Heparan sulfate 3-O-sulfotransferase 1
    • Heparan sulfate D glucosaminyl 3 O sulfotransferase 1
    • Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 1
    • Heparan sulfate glucosamine 3-O-sulfotransferase 1
    • Heparin glucosamine 3 O sulfotransferase
    • HS3S1_HUMAN
    • HS3ST 1
    • Hs3st1
    see all
  • Function
    Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to position 3 of glucosamine residues in heparan. Catalyzes the rate limiting step in the biosynthesis of heparan sulfate (HSact). This modification is a crucial step in the biosynthesis of anticoagulant heparan sulfate as it completes the structure of the antithrombin pentasaccharide binding site.
  • Tissue specificity
    Highly expressed in the brain and kidney and weakly expressed in the heart, lung and placenta.
  • Sequence similarities
    Belongs to the sulfotransferase 1 family.
  • Cellular localization
    Golgi apparatus lumen.
  • Information by UniProt

Images

  • 15% SDS-PAGE analysis of ab171694 (3µg).

References

ab171694 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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