Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionP17516
    • SpeciesHuman
    • SequenceMGSSHHHHHHSSGLVPRGSHMDPKYQRVELNDGHFMPVLGFGTYAPPEVP RNRAVEVTKLAIEAGFRHIDSAYLYNNEEQVGLAIRSKIADGSVKREDIF YTSKLWCTFFQPQMVQPALESSLKKLQLDYVDLYLLHFPMALKPGETPLP KDENGKVIFDTVDLSATWEVMEKCKDAGLAKSIGVSNFNCRQLEMILNKP GLKYKPVCNQVECHPYLNQSKLLDFCKSKDIVLVAHSALGTQRHKLWVDP NSPVLLEDPVLCALAKKHKRTPALIALRYQLQRGVVVLAKSYNEQRIREN IQVFEFQLTSEDMKVLDGLNRNYRYVVMDFLMDHPDYPFSDEY
    • Molecular weight39 kDa including tags
    • Amino acids1 to 343
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab109831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Specific activity is > 700 pmol/min/μg, and is defined as the amount of enzyme that catalyzes the reduction of 1.0 pmole 3-chlorobenzaldehyde in the presence of NADP per minute at pH 8.8 at 25°C.

  • Applications

    Functional Studies

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity> 90 % SDS-PAGE.
    ab109831 was purified using conventional chromatography.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 20% Glycerol, 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% DTT, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • 3 alpha HSD1
    • 3-alpha-HSD1
    • 3-alpha-hydroxysteroid dehydrogenase type I
    • AK1C4_HUMAN
    • AKR1C4
    • Aldo keto reductase family 1 member C4
    • Aldo-keto reductase family 1 member C4
    • CDR
    • Chlordecone reductase
    • DD-4
    • DD4
    • Dihydrodiol dehydrogenase 4
    • HAKRA
    see all
  • FunctionCatalyzes the transformation of the potent androgen dihydrotestosterone (DHT) into the less active form, 5-alpha-androstan-3-alpha,17-beta-diol (3-alpha-diol). Also has some 20-alpha-hydroxysteroid dehydrogenase activity. The biotransformation of the pesticide chlordecone (kepone) to its corresponding alcohol leads to increased biliary excretion of the pesticide and concomitant reduction of its neurotoxicity since bile is the major excretory route.
  • Tissue specificityLiver specific.
  • Sequence similaritiesBelongs to the aldo/keto reductase family.
  • Post-translational
    modifications
    The N-terminus is blocked.
  • Cellular localizationCytoplasm.
  • Information by UniProt

Recombinant human HSD3a protein images

  • 15% SDS-PAGE image, using ab109831 at 3ug

References for Recombinant human HSD3a protein (ab109831)

ab109831 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab109831.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"