The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% SDS-PAGE. Greater than or equal to 98% as determined by SDS-PAGE and HPLC.
Additional notesLyophilized Hsp20 should be stored desiccated below 0degC. Upon reconstitution Hsp20 can be stored at 4degC for 2-7 days. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA) and store aliquots at –20degC or –70degC. Avoid freeze-thaw cycles.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
ENDOTOXIN: Less than 0.1 ng/mg of Hsp20
ReconstitutionReconstitute in water to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted to other aqueous buffers.
epididymis luminal protein 55
Heat shock 20 kDa like protein p20
Heat shock 20 kDa-like protein p20
Heat shock protein alpha crystallin related B6
Heat shock protein beta 6
Heat shock protein beta-6
Heat shock protein, 20-KD
Heat-shock 27-KD protein 6
Sequence similaritiesBelongs to the small heat shock protein (HSP20) family.
Post-translational modificationsThe N-terminus is blocked.
Cellular localizationCytoplasm. Nucleus. Translocates to nuclear foci during heat shock.