Recombinant human Hsp70 protein (ab78427)



  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman


Our Abpromise guarantee covers the use of ab78427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityab78427 has ATPase activity at the time of manufacture of 3.3µM phosphate liberated/hr/µg protein in a 200µl reaction at 37°C (pH7.5) in the presence of 20ul of 1mM ATP using a Malachite Green assay.
  • Applications

    Functional Studies


    Western blot



  • Purity> 90 % SDS-PAGE.
    ab78427 is affinity purified.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

    Preservative: 200mM Imidazole
    Constituents: 10% Glycerol, 20mM Sodium phosphate, 150mM Sodium chloride, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • DnaK type molecular chaperone HSP70 1
    • Epididymis secretory protein Li 103
    • FLJ54303
    • FLJ54370
    • FLJ54392
    • FLJ54408
    • FLJ75127
    • Heat shock 70 kDa protein 1
    • Heat shock 70 kDa protein 1/2
    • Heat shock 70 kDa protein 1A/1B
    • Heat shock 70kDa protein 1A
    • Heat shock 70kDa protein 1B
    • Heat shock induced protein
    • HEL S 103
    • HSP70 1
    • HSP70 1B
    • HSP70 2
    • HSP70-1/HSP70-2
    • HSP70-1A
    • HSP70.1
    • HSP70.1/HSP70.2
    • HSP70I
    • HSP71_HUMAN
    • HSP72
    • HSPA1
    • HSPA1A
    • HSPA1B
    see all
  • FunctionIn cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell.
  • Tissue specificityHSPA1B is testis-specific.
  • Sequence similaritiesBelongs to the heat shock protein 70 family.
  • Cellular localizationCytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt

Recombinant human Hsp70 protein images

  • Lane 1. Hsp70 protein

    Lane 2. Molecular weight ladder

  • Anti-Hsp70 antibody (ab94368) at 1 µg/ml + Recombinant human Hsp70 protein (ab78427) at 0.1 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 90 seconds

References for Recombinant human Hsp70 protein (ab78427)

ab78427 has not yet been referenced specifically in any publications.

Product Wall

When we tested this protein we loaded 10ul of protein solution with stock 0.01ug/ul concentration. (0.1ug/10ul).

We are selling this protein at concentration 0.47ug/ul. We recommend customer using 2ul of 0.47ug/ul and then adding 8ul of diluen...

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The protein showed ˜75 kDa band.

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This protein is produced in E. coli BL21 cells and purified on a nickel column, eluted with imidazole using a his tag. Thus you should be able to use an anti-his tag antibody to detect this protein.