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Our Abpromise guarantee covers the use of ab80369 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Specific Activity : Kd of 11.8 nM. Assay conditions : 95 ul reaction mix (20 mM HEPES pH7.3, 50 mM KCl, 5 mM MgCl2, 20 mM Na2MoO4, 0.01% Triton X- 100, 100 ug/ml bovine serum albumin, 2 mM DTT, 0-30 nM Hsp90alpha) + 5 ul FITCgeldanamycin, incubate for 4 hrs at 4ºC. Fluorescent polarization measured at room temperature (lambda exc=485 nm, lambda em=528 nm).
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Preservative: 1.53% Imidazole
Constituents: 0.462% DTT, 0.709% Tris HCl, 10% Glycerol, 0.725% Sodium chloride, 0.018% Potassium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ab80369 has not yet been referenced specifically in any publications.