Recombinant human Hsp90 beta protein (ab80033)

Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected BTI-TN-5B1-4 cells
  • Amino Acid Sequence
    • SpeciesHuman

Specifications

Our Abpromise guarantee covers the use of ab80033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    1. The reactions are in 33 mM Hepes pH7.2, 30 mM NaCl, 5 mM MgCl2, 1 mM DTT, 1.5 mM ATP in a 100 µl reaction at 37ºC. It is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm.

    2. The protein concentrations (where applicable) were 2 µM Hsp90 (SPR-102 Hsp90 beta), 2 µM Ahsa1 (His tagged human Ahsa1), 4 µM p23 (cleaved human p23), 4 µM HOP (His-tagged human HOP).

    3. Addition of a two-fold excess of p23 reduced Aha1-mediated Hsp90 stimulation by 30% (when using 2 µM Hsp90 and Aha1).

    4. Addition of a two -fold excess of HOP completely eliminated Aha1-mediated ATPase stimulation as well as intrinsic Hsp90 ATPase activity.

  • Applications

    Western blot

    ELISA

    Inhibition Assay

    Functional Studies

    SDS-PAGE

  • Purity> 90 % SDS-PAGE.
    ab80033 is Multi-step chromatographically purified.
  • FormLiquid
  • Additional notesab80033 is endotoxin free. <0.1 EU/ml.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris, 175mM Sodium chloride, 1mM DTT, 0.1mM EDTA, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • 90 kda heat shock protein beta HSP90 beta
    • D6S182
    • FLJ26984
    • Heat shock 84 kDa
    • Heat shock 90kD protein 1, beta
    • Heat shock 90kDa protein 1 beta
    • Heat shock protein 90 alpha family class B member 1
    • Heat shock protein 90 kDa
    • Heat shock protein 90kDa alpha (cytosolic) class B member 1
    • Heat shock protein 90kDa alpha family class B member 1
    • Heat shock protein beta
    • Heat shock protein HSP 90 beta
    • Heat shock protein HSP 90-beta
    • HS90B_HUMAN
    • HSP 84
    • HSP 90
    • HSP 90 b
    • HSP 90b
    • HSP84
    • HSP90 BETA
    • hsp90ab1
    • HSP90B
    • HSPC2
    • HSPCB
    see all
  • FunctionMolecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.
  • Sequence similaritiesBelongs to the heat shock protein 90 family.
  • DomainThe TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins.
  • Post-translational
    modifications
    Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).
    ISGylated.
    S-nitrosylated; negatively regulates the ATPase activity.
  • Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

Recombinant human Hsp90 beta protein images


  • Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 8 minutes

References for Recombinant human Hsp90 beta protein (ab80033)

ab80033 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. The His tags for all four proteins you asked about are all 6X tags. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"