Recombinant Human HSPC142 protein (ab183249)

Overview

  • Product name
    Recombinant Human HSPC142 protein
  • Protein length
    Full length protein

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MGSSHHHHHH SSGLVPRGSH MGSMEVAEPS SPTEEEEEEE EHSAEPRPRT RSNPEGAEDR AVGAQASVGS RSEGEGEAAS ADDGSLNTSG AGPKSWQVPP PAPEVQIRTP RVNCPEKVII CLDLSEEMSL PKLESFNGSK TNALNVSQKM IEMFVRTKHK IDKSHEFALV VVNDDTAWLS GLTSDPRELC SCLYDLETAS CSTFNLEGLF SLIQQKTELP VTENVQTIPP PYVVRTILVY SRPPCQPQFS LTEPMKKMFQ CPYFFFDVVY IHNGTEEKEE EMSWKDMFAF MGSLDTKGTS YKYEVALAGP ALELHNCMAK LLAHPLQRPC QSHASYSLLE EEDEAIEVEA TV
    • Molecular weight
      39 kDa including tags
    • Amino acids
      1 to 329
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab183249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry

    SDS-PAGE

  • Mass spectrometry
    MALDI-TOF
  • Purity
    > 85 % SDS-PAGE.
    ab183249 is purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.0
    Constituents: 10% Glycerol, 0.58% Sodium chloride, 0.32% Tris HCl

General Info

  • Alternative names
    • BABA1_HUMAN
    • babam1
    • BRCA1 A complex subunit MERIT40
    • BRISC and BRCA1-A complex member 1
    • C19orf62
    • FLJ20571
    • HSPC142
    • hypothetical protein LOC29086
    • Mediator of Rap80 interactions and targeting 40 kDa
    • Mediator of RAP80 interactions and targeting subunit of 40 kDa
    • MERIT 40
    • MERIT40
    • NBA1
    • New component of the BRCA1 A complex
    • New component of the BRCA1-A complex
    • Uncharacterized protein C19orf62
    see all
  • Function
    Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it is required for the complex integrity and its localization at DSBs. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. In these 2 complexes, it is probably required to maintain the stability of BRE/BRCC45 and help the 'Lys-63'-linked deubiquitinase activity mediated by BRCC3/BRCC36. component.
  • Sequence similarities
    Belongs to the BABAM1 family.
  • Domain
    The VWFA-like region is similar to the VWFA domain. Its presence reveals similarities between the structure of the 19S proteasome and the BRCA1-A complexes.
  • Cellular localization
    Cytoplasm. Nucleus. Localizes at sites of DNA damage at double-strand breaks.
  • Information by UniProt

Images

  • 15% SDS PAGE analysis of ab183249 (3µg).

References

ab183249 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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