The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The ED50 was determined by a cytotoxicity assay using Human TF1 cells is < 0.01 ng/ml, corresponding to a specific activity of > 100,000,000 units/mg.
% SDS-PAGE. ab125739 is purified by proprietary chromatographic techniques. Purity is greater than 95.0% as determined by RP-HPLC and SDS-PAGE.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C. Store under desiccating conditions.
pH: 7.40 Constituent: 99% PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
It is recommended to reconstitute ab125739 in sterile ultra pure water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Upon reconstitution ab125739 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.