The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityThe ED50 as determined by a cell proliferation assay using NIH3T3 cells is typically 1-6 ng/ml.
% SDS-PAGE. Purity is greater than 97% as determined by HPLC and SDS-PAGE. Endotoxin Level: <0.1 ng/µg.
ab123776 shows higher potency than regular human IGF-I in bioactivity assay. ab123776 was produced from E. coli using Animal Origin Free (AOF) components and therefore it is suitable for bioproduction also.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C. Store under desiccating conditions.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute in dH2O or 10 mM HAC to 1.0 mg/ml. This solution can then be diluted into other buffered solutions or stored at 4°C for 1 week or –20°C for future use. Reconstituted ab123776 should be stored
at working aliquots at -20°C. Avoid freeze/thaw cycles.
Insulin like growth factor 1
Insulin like growth factor 1 (somatomedin C)
Insulin like growth factor IA
Insulin like growth factor IB
Insulin-like growth factor I
Mechano growth factor
FunctionThe insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake.
Involvement in diseaseDefects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.
Sequence similaritiesBelongs to the insulin family.