Additional sequence informationThe sequence of the first five N-terminal amino acids was determined and was found to be Gly-Pro-Glu-Thr-Leu. N-terminal methionine has been completely removed enzymatically.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The ED50 as determined by a cell proliferation assay using murine BALB/C 3T3 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1×106 units/mg.
% SDS-PAGE. ab73455 is purified by proprietary chromatographic techniques.
Purity is greater than 98.0% as determined by RP-HPLC and SDS-PAGE.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 50mM Acetic acid
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Insulin like growth factor 1
Insulin like growth factor 1 (somatomedin C)
Insulin like growth factor IA
Insulin like growth factor IB
Insulin-like growth factor I
Mechano growth factor
FunctionThe insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake.
Involvement in diseaseDefects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.
Sequence similaritiesBelongs to the insulin family.
References for Recombinant human IGF1 protein (ab73455)
This product has been referenced in:
Jaaks P et al. The proprotein convertase furin is required to maintain viability of alveolar rhabdomyosarcoma cells. Oncotarget7:76743-76755 (2016).
Read more (PubMed: 27572312) »