The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The ED50, calculated by the dose-dependant proliferation of murine BALB\C 3T3 cells (measured by 3H-thymidine uptake) is < 1ng/ml, corresponding to a specific activity of 1,000,000units/mg. For most in-vitro applications, ab73457 exerts its biological activity in the concentration range of 0.2-20 ng/ml, corresponding to a specific activity of 50,000-5,000,000units/mg.
% SDS-PAGE. This protein was purified by proprietary chromatographic techniques.
Purity: Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 50mM Acetic acid
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Reconstitute in 100mM Acetic acid at not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Insulin like growth factor 1
Insulin like growth factor 1 (somatomedin C)
Insulin like growth factor IA
Insulin like growth factor IB
Insulin-like growth factor I
Mechano growth factor
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake.
Involvement in disease
Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.