Recombinant human IGF1 Receptor beta subunit protein (ab80349)
Key features and details
- Expression system: Insect cells
- Purity: > 80% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human IGF1 Receptor beta subunit protein -
Biological activity
Specific activity: ~100 nmol/min/mg at 25°C using poly(Glu:Tyr)4:1 substrate. Reaction conditions are 50 mM Tris, pH 7.5, 5 mM MgCl2, 5 mM MnCl2, 1 mM DTT, 250 µM ATP. The kinase concentration was 0.5-1 µM and poly-L-lysine was added in equimolar amounts to the kinase.
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Purity
> 80 % SDS-PAGE.
Affinity purified. -
Expression system
Insect cells -
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Amino acids
930 to 1337
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Specifications
Our Abpromise guarantee covers the use of ab80349 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.605% TrisThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- CD221
- CD221 antigen
- EC 2.7.10.1
see all -
Function
This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present in a hybrid receptor with INSR, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed:16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin. -
Tissue specificity
Found as a hybrid receptor with INSR in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta (at protein level). Expressed in a variety of tissues. -
Involvement in disease
Defects in IGF1R are a cause of insulin-like growth factor 1 resistance (IGF1RES) [MIM:270450]. It is a disorder characterized by intrauterine growth retardation and poor postnatal growth accompanied with increased plasma IGF1. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 3 fibronectin type-III domains.
Contains 1 protein kinase domain. -
Post-translational
modificationsThe cytoplasmic domain of the beta subunit is autophosphorylated on tyrosine residues in response to insulin-like growth factor I (IGF I).
Phosphorylation of Tyr-980 is required for IRS1- and SHC1-binding. -
Cellular localization
Membrane. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab80349 has not yet been referenced specifically in any publications.