Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionP01344
    • SpeciesHuman
    • SequenceAYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE
    • Molecular weight8 kDa

Associated products

Specifications

Our Abpromise guarantee covers the use of ab9575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    The ED50 was determined by a cell proliferation assay using FDC-P1 cells is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg.
  • Applications

    Functional Studies

    SDS-PAGE

  • FormLyophilised
  • Additional notes

    The ED50 was determined by a cell proliferation assay using FDC-P1 cells is < 2.0 ng/ml, corresponding to a specific activity of >5 x 105 units/mg.

    ab9575 was used as a positive control for ab9574 (Anti-IGF2 antibody).

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    n/a

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionReconstitute with 100ul of water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.

General Info

  • Alternative names
    • C11orf43
    • IGF 2
    • IGF II
    • IGF-II
    • IGF2
    • IGF2_HUMAN
    • IGFII
    • INSIGF
    • Insulin like Growth Factor 2
    • insulin like growth factor 2 (somatomedin A)
    • Insulin like growth factor II
    • Insulin like growth factor II precursor
    • Insulin like growth factor type 2
    • pp9974
    • Preptin
    • putative insulin like growth factor II associated protein
    • Somatomedin A
    • Somatomedin-A
    see all
  • FunctionThe insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
    Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.
  • Involvement in diseaseEpigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SIRS) [MIM:180860]. SIRS is a clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations.
  • Sequence similaritiesBelongs to the insulin family.
  • Post-translational
    modifications
    O-glycosylated with a core 1 or possibly core 8 glycan.
  • Cellular localizationSecreted.
  • Information by UniProt

Recombinant human IGF2 protein images

  • Standard curve for IGF2 (Analyte: ab9575); dilution range 1pg/ml to 1µg/ml using Capture Antibody ab86485 at 1µg/ml and Detector Antibody ab18954 at 0.5µg/ml.

References for Recombinant human IGF2 protein (ab9575)

This product has been referenced in:
  • Brouwer-Visser J  et al. Insulin-like growth factor 2 silencing restores taxol sensitivity in drug resistant ovarian cancer. PLoS One 9:e100165 (2014). Functional Studies . Read more (PubMed: 24932685) »
  • Suvasini R  et al. Insulin Growth Factor-2 Binding Protein 3 (IGF2BP3) Is a Glioblastoma-specific Marker That Activates Phosphatidylinositol 3-Kinase/Mitogen-activated Protein Kinase (PI3K/MAPK) Pathways by Modulating IGF-2. J Biol Chem 286:25882-90 (2011). Read more (PubMed: 21613208) »

See all 2 Publications for this product

Product Wall

We have several IGF2 antibodies but the immunogens for each are not all commercially available, and not all are raised against peptides. For the example I gave, ab9574, the immunogen is full-length recombinant IGF2, and this is available as ab9575: Read More

The blocking protein would be ab9575. Please see datasheet for specifics.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"